Document Detail


Capacity of circulating neutrophils to produce reactive oxygen species after exhaustive exercise.
MedLine Citation:
PMID:  8889756     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To investigate the cause of disagreement within the large body of literature concerning the effect of exercise on the capacity of circulating neutrophils to produce reactive oxygen species (ROS), 10 male endurance-trained athletes underwent maximal exercise. The generation of superoxide radical (O2-.) by neutrophils was first detected on a cell-by-cell basis by using histochemical nitro blue tetrazolium tests performed directly on fresh unseparated blood, which showed that responsive neutrophils under several stimulatory conditions relatively decreased after exercise. Similarly, O2-. detected with bis-N-methylacridinium nitrate (lucigenin)-dependent chemiluminescence (CL) of a fixed number of purified neutrophils on stimulation with opsonized zymosan was decreased slightly after exercise. In contrast, the 5-amino-2,3-dihydro-1,4-phthalazinedione (luminol)-dependent CL response of the neutrophils indicative of the myeloperoxidase (MPO)-mediated formation of highly reactive oxidants was significantly enhanced after exercise. It therefore suggests that the pathway of neutrophil ROS metabolism might be forwarded from the precursor O2-. production to the stages of more reactive oxidant formation due to the facilitation of MPO degranulation. In addition, these phenomena were closely associated with the exercise-induced mobilization of neutrophils from the marginated pool into the circulation, which was mediated by the overshooting of catecholamines during exercise. These findings indicate that the use of different techniques for detecting ROS or the different stages of neutrophil ROS metabolism could explain some of the disparate findings of the previous studies.
Authors:
K Suzuki; H Sato; T Kikuchi; T Abe; S Nakaji; K Sugawara; M Totsuka; K Sato; K Yamaya
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of applied physiology (Bethesda, Md. : 1985)     Volume:  81     ISSN:  8750-7587     ISO Abbreviation:  J. Appl. Physiol.     Publication Date:  1996 Sep 
Date Detail:
Created Date:  1997-02-07     Completed Date:  1997-02-07     Revised Date:  2013-09-26    
Medline Journal Info:
Nlm Unique ID:  8502536     Medline TA:  J Appl Physiol (1985)     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1213-22     Citation Subset:  IM    
Affiliation:
Department of Hygiene, Hirosaki University School of Medicine, Aomori, Japan.
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MeSH Terms
Descriptor/Qualifier:
Adult
Exercise / physiology*
Heart Rate / physiology*
Histocytochemistry
Humans
Male
Neutrophils / metabolism*
Pulmonary Ventilation / physiology*
Reactive Oxygen Species / metabolism*
Chemical
Reg. No./Substance:
0/Reactive Oxygen Species

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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