Document Detail


Capacity of ascorbyl palmitate to produce the ascorbyl radical in vitro: an electron spin resonance investigation.
MedLine Citation:
PMID:  15209539     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This study aims to compare the electron spin resonance (ESR) spectra emitted by human blood loaded with either ascorbyl-6-palmitate (AP), a lipid-soluble derivative of ascorbic acid (AA), or with AA. Whole blood of a healthy male individual was equilibrated with equimolar concentrations of AP and AA of 200, 400, and 800 micromol/l. The intensity of the ESR signal, expressed as the peak-to-peak amplitude, reflects the amount of unpaired spins that are created due to the reducing action of AA and is proportional, in relative terms, to the amount of the ascorbyl radical formed. We found that the blood with AP emitted an ESR signal whose singlet shape, width, and location precisely correlate with the known characteristics of the ascorbyl radical in vitro. The signal magnitude increased linearly with increasing concentrations of AP and was similar to that of AA. We conclude that AP is biologically active, as it generates the ascorbyl radical, an action that also underlies the scavenging process by ascorbic acid. To this end, ascorbyl-6-palmitate might have potential advantages, due to its ability to penetrate biomembranes and to act at the lipid-related molecular target sites.
Authors:
M Pokorski; B Gonet
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Physiological research / Academia Scientiarum Bohemoslovaca     Volume:  53     ISSN:  0862-8408     ISO Abbreviation:  Physiol Res     Publication Date:  2004  
Date Detail:
Created Date:  2004-06-22     Completed Date:  2005-01-11     Revised Date:  2008-04-02    
Medline Journal Info:
Nlm Unique ID:  9112413     Medline TA:  Physiol Res     Country:  Czech Republic    
Other Details:
Languages:  eng     Pagination:  311-6     Citation Subset:  IM    
Affiliation:
Department of Respiratory Physiology, Medical Research Center, Polish Academy of Sciences, Warshaw, Poland. mpokorski@cmdik.pan.pl
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MeSH Terms
Descriptor/Qualifier:
Adult
Ascorbic Acid / analogs & derivatives*,  chemistry,  metabolism*
Blood / metabolism*
Blood Chemical Analysis / methods
Dehydroascorbic Acid / analogs & derivatives*,  chemistry,  metabolism*
Electron Spin Resonance Spectroscopy / methods*
Humans
Male
Molecular Conformation
Chemical
Reg. No./Substance:
137-66-6/6-O-palmitoylascorbic acid; 490-83-5/Dehydroascorbic Acid; 50-81-7/Ascorbic Acid; 6730-29-6/semidehydroascorbic acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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