| Capacitation and acrosome reactions in human spermatozoa monitored by a chlortetracycline fluorescence assay. | |
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MedLine Citation:
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PMID: 3115838 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Human spermatozoa were incubated in culture medium containing human serum albumin (HSA) to promote capacitation, which was monitored by a rapid chlortetracycline (CTC) fluorescence assay. Four CTC fluorescence patterns were readily distinguished, one of which appeared to be correlated with capacitated sperm. When capacitated sperm were treated with either ionophore A23187 or acid-solubilized mouse zonae pellucidae to induce the acrosome reaction, the CTC assay identified acrosome-reacted sperm by lack of fluorescence on the head. Fresh sperm would not undergo the induced acrosome reaction. The percentages of acrosome-reacted sperm identified by the CTC assay in induced and control populations were the same as those identified by the presently used indirect immunofluorescence and triple stain assays. |
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Authors:
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M A Lee; G S Trucco; K B Bechtol; N Wummer; G S Kopf; L Blasco; B T Storey |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Fertility and sterility Volume: 48 ISSN: 0015-0282 ISO Abbreviation: Fertil. Steril. Publication Date: 1987 Oct |
Date Detail:
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Created Date: 1987-11-06 Completed Date: 1987-11-06 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0372772 Medline TA: Fertil Steril Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 649-58 Citation Subset: IM |
Affiliation:
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University of Pennsylvania School of Medicine, Philadelphia 19104-6080. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acrosome
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physiology* Animals Calcimycin / pharmacology Chlortetracycline* Female Fluorescent Antibody Technique Humans Kinetics Male Mice Microscopy, Fluorescence* Serum Albumin / pharmacology Sperm Capacitation* / drug effects Spermatozoa / physiology* Zona Pellucida / physiology |
| Grant Support | |
ID/Acronym/Agency:
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GM-23892/GM/NIGMS NIH HHS; HD-06274/HD/NICHD NIH HHS; HD-19096/HD/NICHD NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Serum Albumin; 52665-69-7/Calcimycin; 57-62-5/Chlortetracycline |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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