Document Detail

Capacitation and the acrosome reaction in sperm from men with various semen profiles monitored by a chlortetracycline fluorescence assay.
MedLine Citation:
PMID:  1544697     Owner:  NLM     Status:  MEDLINE    
Sperm obtained from groups of men with various semen profiles were incubated for 8 h in BWW medium containing human serum albumin to promote capacitation. Capacitation and the acrosome reaction were monitored by a chlortetracycline (CTC) fluorescence assay. Four distinct CTC patterns were observed on the sperm head. No significant difference was observed in the time-course curve of these CTC patterns in sperm obtained from normozoospermic, asthenozoospermic and oligozoospermic men. Spontaneous and A23187-induced acrosome reactions were also comparable in these groups. However, in sperm obtained from teratozoospermic and polyzoospermic men, the increase in CTC pattern associated with capacitation appeared slower and sluggish. In these two groups, the induced acrosome reaction was also significantly lower when compared to that in the other three groups of men. In polyzoospermia, the spontaneous acrosome reaction was significantly lower when compared to all the other groups. Fresh sperm would not undergo the acrosome reaction following A23187 treatment. The results of this study indicate sluggish (defective) capacitation and inability of capacitated sperm to undergo induced acrosome reaction in teratozoospermic and polyzoospermic men as evaluated by the CTC method.
S D Kholkute; P Meherji; C P Puri
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  International journal of andrology     Volume:  15     ISSN:  0105-6263     ISO Abbreviation:  Int. J. Androl.     Publication Date:  1992 Feb 
Date Detail:
Created Date:  1992-04-15     Completed Date:  1992-04-15     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  8000141     Medline TA:  Int J Androl     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  43-53     Citation Subset:  IM    
Institute for Research in Reproduction (ICMR), Parel, Bombay, India.
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MeSH Terms
Acrosome / physiology*
Calcimycin / pharmacology
Evaluation Studies as Topic
Infertility, Male / physiopathology
Microscopy, Fluorescence
Sperm Capacitation*
Reg. No./Substance:
52665-69-7/Calcimycin; 57-62-5/Chlortetracycline

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