| Capacitance-based assay for real-time monitoring of endocytosis and cell viability. | |
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MedLine Citation:
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PMID: 22522711 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Label-free cell-based assays have emerged as a promising means for high-throughput screening. Most label-free sensors are based on impedance measurements that reflect the passive electrical properties of cells. Here we introduce a capacitance-based assay that measures the dielectric constant (capacitance) of biological cells, and demonstrate the feasibility of analyzing endocytosis and screening chemotherapeutic agents with this assay. Endocytosis induces a change in the zeta potential, leading to a change in the dielectric constant which enables real-time endocytosis monitoring using the capacitance sensor. Additionally, since the dielectric constant is proportional to cell radius and cell volume, cell viability can be estimated from the change in capacitance. Therefore, the capacitance sensor array can also be used for cytotoxicity testing for large-scale chemotherapeutic screening. |
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Authors:
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Rimi Lee; Jihun Kim; Sook Young Kim; Seon Mi Jang; Sun-Mi Lee; In-Hong Choi; Seung Woo Park; Jeon-Soo Shin; Kyung-Hwa Yoo |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2012-04-20 |
Journal Detail:
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Title: Lab on a chip Volume: 12 ISSN: 1473-0189 ISO Abbreviation: Lab Chip Publication Date: 2012 Jul |
Date Detail:
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Created Date: 2012-06-08 Completed Date: 2012-10-03 Revised Date: 2012-10-12 |
Medline Journal Info:
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Nlm Unique ID: 101128948 Medline TA: Lab Chip Country: England |
Other Details:
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Languages: eng Pagination: 2377-84 Citation Subset: IM |
Affiliation:
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Graduate Program for Nanomedical Science and Technology, Yonsei University, Seoul 120-749, Republic of Korea. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Antibodies, Monoclonal
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immunology Antineoplastic Agents / toxicity Cell Line, Tumor Cell Size Cell Survival / drug effects* Electric Capacitance* Electrochemical Techniques / instrumentation, methods Endocytosis / drug effects* Humans Metal Nanoparticles / chemistry Phagocytosis Pinocytosis Receptor, erbB-2 / immunology, metabolism Silver / chemistry |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Monoclonal; 0/Antineoplastic Agents; 7440-22-4/Silver; EC 2.7.10.1/ERBB2 protein, human; EC 2.7.10.1/Receptor, erbB-2 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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