Document Detail


Capacitance-based assay for real-time monitoring of endocytosis and cell viability.
MedLine Citation:
PMID:  22522711     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Label-free cell-based assays have emerged as a promising means for high-throughput screening. Most label-free sensors are based on impedance measurements that reflect the passive electrical properties of cells. Here we introduce a capacitance-based assay that measures the dielectric constant (capacitance) of biological cells, and demonstrate the feasibility of analyzing endocytosis and screening chemotherapeutic agents with this assay. Endocytosis induces a change in the zeta potential, leading to a change in the dielectric constant which enables real-time endocytosis monitoring using the capacitance sensor. Additionally, since the dielectric constant is proportional to cell radius and cell volume, cell viability can be estimated from the change in capacitance. Therefore, the capacitance sensor array can also be used for cytotoxicity testing for large-scale chemotherapeutic screening.
Authors:
Rimi Lee; Jihun Kim; Sook Young Kim; Seon Mi Jang; Sun-Mi Lee; In-Hong Choi; Seung Woo Park; Jeon-Soo Shin; Kyung-Hwa Yoo
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-04-20
Journal Detail:
Title:  Lab on a chip     Volume:  12     ISSN:  1473-0189     ISO Abbreviation:  Lab Chip     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-06-08     Completed Date:  2012-10-03     Revised Date:  2012-10-12    
Medline Journal Info:
Nlm Unique ID:  101128948     Medline TA:  Lab Chip     Country:  England    
Other Details:
Languages:  eng     Pagination:  2377-84     Citation Subset:  IM    
Affiliation:
Graduate Program for Nanomedical Science and Technology, Yonsei University, Seoul 120-749, Republic of Korea.
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MeSH Terms
Descriptor/Qualifier:
Antibodies, Monoclonal / immunology
Antineoplastic Agents / toxicity
Cell Line, Tumor
Cell Size
Cell Survival / drug effects*
Electric Capacitance*
Electrochemical Techniques / instrumentation,  methods
Endocytosis / drug effects*
Humans
Metal Nanoparticles / chemistry
Phagocytosis
Pinocytosis
Receptor, erbB-2 / immunology,  metabolism
Silver / chemistry
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Antineoplastic Agents; 7440-22-4/Silver; EC 2.7.10.1/ERBB2 protein, human; EC 2.7.10.1/Receptor, erbB-2

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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