Document Detail


Cantharidin induces apoptosis in human bladder cancer TSGH 8301 cells through mitochondria-dependent signal pathways.
MedLine Citation:
PMID:  20878071     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cantharidin has shown potent anticancer activities on many types of human cancer cells. This study was performed to elucidate whether mitochondria and caspases are involved in the modulation of apoptosis and cell cycle arrest by cantharidin in human bladder cancer cells. The effect of cantharidin on cell cycle arrest, apoptosis, caspases, reactive oxygen species (ROS) and mitochondrial membrane potential (ΔΨ(m)) were measured by flow cytometry, and the levels of apoptosis-associated proteins and its regulatory molecules were studied by Western blotting. Cantharidin-induced apoptosis and DNA damage was determined by flow cytometric analysis, DAPI staining and Comet assay. After cantharidin treatment, the active forms of caspase-3, -8 and -9 were promoted. Cantharidin-induced apoptosis was associated with enhanced ROS and Ca(2+) generations, caused DNA damage, decreased the levels of ΔΨ(m) and promoted Endo G and AIF released from mitochondria. Cantharidin-induced G0/G1 arrest was associated with a marked decrease in the protein expressions of cyclin E and Cdc25c but promoted the levels of p21 and p-p53. Cantharidin-induced apoptosis was accompanied with up-regulation of the protein expression of Bax and PARP, but down-regulation of the protein levels of Bcl-2, resulting in dysfunction of mitochondria then led to Endo G and AIF release for causing induction of apoptosis.
Authors:
Jehn-Hwa Kuo; Yung-Lin Chu; Jai-Sing Yang; Jing-Pin Lin; Kuang-Chi Lai; Hsiu-Maan Kuo; Te-Chun Hsia; Jing-Gung Chung
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  International journal of oncology     Volume:  37     ISSN:  1791-2423     ISO Abbreviation:  Int. J. Oncol.     Publication Date:  2010 Nov 
Date Detail:
Created Date:  2010-09-29     Completed Date:  2011-01-25     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9306042     Medline TA:  Int J Oncol     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  1243-50     Citation Subset:  IM    
Affiliation:
Special Class of Healthcare, Industry Management, Central Taiwan University of Science and Technology, Taichung, Taiwan, ROC.
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MeSH Terms
Descriptor/Qualifier:
Antineoplastic Agents / pharmacology*
Apoptosis / drug effects
Blotting, Western
Cantharidin / pharmacology*
Cell Line, Tumor
Cell Separation
Comet Assay
Flow Cytometry
Humans
Membrane Potential, Mitochondrial / drug effects
Microscopy, Confocal
Mitochondria / drug effects*
Reactive Oxygen Species / metabolism
Signal Transduction / drug effects*
Urinary Bladder Neoplasms / metabolism,  pathology*
Chemical
Reg. No./Substance:
0/Antineoplastic Agents; 0/Reactive Oxygen Species; 56-25-7/Cantharidin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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