| Canine urinary bladder epithelial cells: preparation for cell culture by enzyme dispersion. | |
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MedLine Citation:
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PMID: 195376 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In a qualitative and quantitative study of enzymic dispersion of cells from the mucosal layer stripped from canine urinary bladder, trypsin was found to be equal or superior to the other enzymes tested for dispersal of urothelial cells specifically. Collagenase or collagenase plus trypsin served to disperse the whole tissue. A procedure for recovering the urothelial cells as a single-cell suspension and establishing them in culture is presented. The morphology, culture behaviour, and chromosome complement of these cells is described. |
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Authors:
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R A Bonar; C F Reich; Y Sharief |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Urological research Volume: 5 ISSN: 0300-5623 ISO Abbreviation: Urol. Res. Publication Date: 1977 |
Date Detail:
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Created Date: 1977-08-12 Completed Date: 1977-08-12 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0364311 Medline TA: Urol Res Country: GERMANY, WEST |
Other Details:
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Languages: eng Pagination: 87-94 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Culture Techniques Dogs Epithelial Cells Epithelium / enzymology Microbial Collagenase / metabolism Trypsin / metabolism Urinary Bladder / enzymology*, pathology |
| Chemical | |
Reg. No./Substance:
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EC 3.4.21.4/Trypsin; EC 3.4.24.3/Microbial Collagenase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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