Document Detail


Canine serum thermostable alkaline phosphatase isoenzyme from a dog with hepatocellular carcinoma.
MedLine Citation:
PMID:  17085898     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A dog histopathologically diagnosed with hepatocellular carcinoma (HCC) showed very high serum alkaline phosphatase (ALP) activity. A supernatant of ascitic fluid and tumor tissue extracted from the dog also showed much higher ALP activity than normal. ALP isoenzyme analysis of samples was performed using polyacrylamide gel disk electrophoresis, and a wide, broad abnormal band was observed. By various treatments, the abnormal band showed thermostability, which is a characteristic of tumor-associated ALP that has only been reported in humans. The thermostable ALP isoenzyme was not found in sera from 39 dogs with several types of tumor that originated from the liver, except for HCC, nor was it found in 10 dogs with hepatic diseases that did not include hepatic tumors. The thermostable ALP isoenzyme seemed to be associated with canine HCC.
Authors:
Yu-ichi Fukui; Jun Sato; Reeko Sato; Jun Yasuda; Yoshihisa Naito
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Publication Detail:
Type:  Case Reports; Journal Article    
Journal Detail:
Title:  The Journal of veterinary medical science / the Japanese Society of Veterinary Science     Volume:  68     ISSN:  0916-7250     ISO Abbreviation:  J. Vet. Med. Sci.     Publication Date:  2006 Oct 
Date Detail:
Created Date:  2006-11-06     Completed Date:  2006-12-14     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9105360     Medline TA:  J Vet Med Sci     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  1129-32     Citation Subset:  IM    
Affiliation:
Department of Veterinary Medicine, Faculty of Agriculture, Iwate University.
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MeSH Terms
Descriptor/Qualifier:
Alkaline Phosphatase / blood*
Animals
Carcinoma, Hepatocellular / enzymology*,  pathology,  veterinary*
Dog Diseases / enzymology*,  pathology
Dogs
Electrophoresis, Polyacrylamide Gel / veterinary
Enzyme Stability
Fatal Outcome
Female
Hot Temperature
Isoenzymes / blood
Liver Neoplasms / enzymology*,  pathology,  veterinary*
Chemical
Reg. No./Substance:
0/Isoenzymes; EC 3.1.3.1/Alkaline Phosphatase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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