Document Detail

Canine bone marrow cells differentiate into hepatocyte-like cells and placental hydrolysate is a potential inducer.
MedLine Citation:
PMID:  19121529     Owner:  NLM     Status:  MEDLINE    
Hepatocyte growth factor (HGF) can stimulate human and rat bone marrow (BM) cells to differentiate into hepatocytes. A human placental hydrolysate (hPH) stimulates proliferation of hepatocytes, but its role as a potential inducer of BM cells to form hepatocytes is unclear. To determine if canine BM cells stimulated with HGF or hPH differentiate into hepatocyte-like cells, BM cells were cultured with HGF or hPH. The cultured cells underwent morphological examination, expression of albumin and cytokeratin 18 (CK18), hepatic function tests including uptake of low-density lipoprotein (LDL) and cytochrome P (CYP) 450 activity. Albumin mRNA and protein expression of albumin and CK18 proteins were detected in cultures with HGF and hPH. Furthermore, these cells demonstrated LDL uptake and CYP450 activity. These results indicate that canine BM cells can differentiate into hepatocyte-like cells when stimulated by both HGF and that hPH may be an effective inducer of hepatic differentiation.
Sakurako Neo; Takefumi Ishikawa; Kikumi Ogiwara; Norio Kansaku; Miyuki Nakamura; Masashi Watanabe; Masaharu Hisasue; Ryo Tsuchiya; Takatsugu Yamada
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-01-01
Journal Detail:
Title:  Research in veterinary science     Volume:  87     ISSN:  1532-2661     ISO Abbreviation:  Res. Vet. Sci.     Publication Date:  2009 Aug 
Date Detail:
Created Date:  2009-05-18     Completed Date:  2009-09-04     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0401300     Medline TA:  Res Vet Sci     Country:  England    
Other Details:
Languages:  eng     Pagination:  1-6     Citation Subset:  IM    
Laboratory of Veterinary Internal Medicine II, Department of Veterinary Medicine, Azabu University, 1-17-71 Fuchinobe, Sagamihara City, Kanagawa 229-8501, Japan.
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MeSH Terms
Albumins / genetics,  metabolism
Bone Marrow Cells / cytology*,  drug effects*
Cells, Cultured
Cytochrome P-450 Enzyme System / metabolism
Gene Expression Regulation / drug effects
Hepatocyte Growth Factor / pharmacology*
Hepatocytes / cytology,  physiology*
Lipoproteins, LDL / metabolism
Proto-Oncogene Proteins c-met / metabolism
RNA, Messenger / genetics,  metabolism
Reg. No./Substance:
0/Albumins; 0/Lipoproteins, LDL; 0/RNA, Messenger; 67256-21-7/Hepatocyte Growth Factor; 9035-51-2/Cytochrome P-450 Enzyme System; EC Proteins c-met

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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