Document Detail

Canine MDCK cell lines are refractory to infection with human and mouse prions.
MedLine Citation:
PMID:  18423803     Owner:  NLM     Status:  MEDLINE    
Influenza vaccine production in embryonated eggs is associated with many disadvantages, and production in cell culture systems is a viable alternative. Madin Darby canine kidney (MDCK) cells are permissive for a variety of orthomyxoviruses and have proven particularly suitable for vaccine mass production. However, mammalian cells harboring the Prnp gene can theoretically acquire prion infections. Here, we have attempted to infect MDCK cells and substrains thereof with prions. We found that MDCK cells did not produce any protease-resistant PrP(Sc) upon exposure to brain homogenates derived from humans suffering from Creutzfeldt-Jakob disease (CJD) or from mice infected with Rocky Mountain Laboratory (RML) scrapie prions. Further, transmission of MDCK lysates to N2aPK1 cells did not induce formation of PrP(Sc) in the latter. PrP(C) biogenesis and processing in MDCK cells were similar to those of prion-sensitive N2aPK1 cells. However, steady-state levels of PrP(C) were very low, and PrP(C) did not partition with detergent-resistant membranes upon density gradient analysis. These factors may account for their resistance to infection. Alternatively, prion resistance may be related to the specific sequence of canine Prnp, as suggested by the lack of documented prion diseases in dogs.
Magdalini Polymenidou; Heidi Trusheim; Lena Stallmach; Rita Moos; Christian Julius; Gino Miele; Claudia Lenz-Bauer; Adriano Aguzzi
Related Documents :
87503 - Adherence of multiple sclerosis lymphocytes to measles infected cells.
17989223 - Cell division modulates prion accumulation in cultured cells.
23468593 - Hedgehog signaling acts with the temporal cascade to promote neuroblast cell cycle exit.
Publication Detail:
Type:  Journal Article     Date:  2008-04-09
Journal Detail:
Title:  Vaccine     Volume:  26     ISSN:  0264-410X     ISO Abbreviation:  Vaccine     Publication Date:  2008 May 
Date Detail:
Created Date:  2008-05-09     Completed Date:  2008-07-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8406899     Medline TA:  Vaccine     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  2601-14     Citation Subset:  IM    
Institute of Neuropathology, University Hospital of Zürich, Schmelzbergstrasse 12, Zürich, Switzerland.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Amino Acid Sequence
Cell Line*
Creutzfeldt-Jakob Syndrome
Molecular Sequence Data
PrPC Proteins / chemistry
PrPSc Proteins / chemistry
Prions / pathogenicity*
Sequence Alignment
Reg. No./Substance:
0/PrPC Proteins; 0/PrPSc Proteins; 0/Prions

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Mitochondrial relationships and divergence dates of the African colobines: evidence of Miocene origi...
Next Document:  Addition of CpG ODN to recombinant Pseudomonas aeruginosa ExoProtein A conjugates of AMA1 and Pfs25 ...