| Candidacidal activities of the glucose oxidase-mediated lactoperoxidase system. | |
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MedLine Citation:
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PMID: 22137328 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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OBJECTIVE: The purpose of this study was to investigate the candidacidal activity of the glucose oxidase-mediated lactoperoxidase system at various levels of glucose and glucose oxidase. DESIGN: Candida albicans ATCC strains 18804, 10231, 11006, bovine lactoperoxidase (25 and 50μg/mL), and KSCN (1mM) were used. Different levels of glucose oxidase (1, 5, 10, and 20units/mL) and glucose (0.03, 0.3, and 3.0mg/mL) were added to complete the system. The candidacidal activity of the system was examined by preincubating its components for 0-60min, and then with C. albicans. Candidacidal activity was determined by comparing the numbers of CFU and calculating the percent loss of cell viability. RESULTS: The system displayed 13.9-27.4% (without preincubation) to 28.6-34.3% (preincubation for 60min) loss of viability at 25μg/mL of bovine lactoperoxidase, 10units/mL of glucose oxidase, and 0.03mg/mL of glucose; similar results were obtained with 20units/mL of glucose oxidase or 0.06mg/mL of glucose. The candidacidal activity of the system increased markedly as the glucose concentration increased. The candidacidal activity displayed 87.2% (without preincubation) to 100.0% (preincubation for 60min) at 3.0mg/mL of glucose. At 3.0mg/mL of glucose, the system containing 1 or 5units of glucose oxidase also showed significant levels of candidacidal activity. CONCLUSIONS: The candidacidal activity of the glucose oxidase-mediated lactoperoxidase system at physiological concentrations of salivary glucose was moderate, but was greatly elevated with increases of glucose level. |
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Authors:
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Hong-Seop Kho; Yoon-Young Kim; Ji-Youn Chang; Moon-Jong Kim; Sang-Goo Lee |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-11-30 |
Journal Detail:
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Title: Archives of oral biology Volume: - ISSN: 1879-1506 ISO Abbreviation: - Publication Date: 2011 Nov |
Date Detail:
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Created Date: 2011-12-5 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0116711 Medline TA: Arch Oral Biol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2011 Elsevier Ltd. All rights reserved. |
Affiliation:
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Dept. of Oral Medicine and Oral Diagnosis, School of Dentistry and Dental Research Institute, Seoul National University, Yunkeun-Dong 28, Chongro-Ku, Seoul 110-749, Republic of Korea. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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