| Candida species differ in their interactions with immature human gastrointestinal epithelial cells. | |
| | |
MedLine Citation:
|
PMID: 21283049 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Life-threatening gastrointestinal (GI) diseases of prematurity are highly associated with systemic candidiasis. This implicates the premature GI tract as an important site for invasion by Candida. Invasive interactions of Candida spp. with immature enterocytes have heretofore not been analyzed. Using a primary immature human enterocyte line, we compared the ability of multiple isolates of different Candida spp. to penetrate, injure, and induce a cytokine response from host cells. Of all the Candida spp. analyzed, C. albicans had the greatest ability to penetrate and injure immature enterocytes and to elicit IL-8 release (p < 0.01). In addition, C. albicans was the only Candida spp. to form filamentous hyphae when in contact with immature enterocytes. Similarly, a C. albicans mutant with defective hyphal morphogenesis and invasiveness had attenuated cytotoxicity for immature enterocytes (p < 0.003). Thus, hyphal morphogenesis correlates with immature enterocyte penetration, injury, and inflammatory responses. Furthermore, variability in enterocyte injury was observed among hyphal-producing C. albicans strains, suggesting that individual organism genotypes also influence host-pathogen interactions. Overall, the finding that Candida spp. differed in their interactions with immature enterocytes implicates that individual spp. may use different pathogenesis mechanisms. |
| | |
Authors:
|
Christina Falgier; Sara Kegley; Heather Podgorski; Timothy Heisel; Kathleen Storey; Catherine M Bendel; Cheryl A Gale |
Related Documents
:
|
15129319 - Role of extracellular adenosine triphosphate in human skin. 20605059 - Interleukin-1, inflammasomes and the skin. 18323789 - Critical role of hif-1alpha in keratinocyte defense against bacterial infection. 17224449 - Uva induces granzyme b in human keratinocytes through mif: implication in extracellular... 12088639 - Keratinocytes and cytokines. 21067579 - Transcriptomic analysis of the temporal host response to skin infestation with the ecto... 10453079 - Analysis of redox regulation of cytochrome c-induced apoptosis in a cell-free system. 10207939 - Cells and cytokines involved in the pathogenesis of sarcoidosis. 21518319 - Chondrocyte death by apoptosis is associated with the initiation and severity of articu... |
Publication Detail:
|
Type: Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Pediatric research Volume: 69 ISSN: 1530-0447 ISO Abbreviation: Pediatr. Res. Publication Date: 2011 May |
Date Detail:
|
Created Date: 2011-04-15 Completed Date: 2011-07-25 Revised Date: 2012-05-02 |
Medline Journal Info:
|
Nlm Unique ID: 0100714 Medline TA: Pediatr Res Country: United States |
Other Details:
|
Languages: eng Pagination: 384-9 Citation Subset: IM |
Affiliation:
|
Department of Pediatrics, University of Minnesota Medical School, Minneapolis, Minnesota, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Bacterial Adhesion Candida / classification, genetics, pathogenicity* Cell Line Enterocytes / immunology, microbiology*, pathology Genotype Host-Pathogen Interactions* / genetics Humans Hyphae Inflammation Mediators / metabolism Interleukin-8 / metabolism Mutation Phenotype |
| Grant Support | |
ID/Acronym/Agency:
|
AI057440/AI/NIAID NIH HHS; R01 AI057440-04/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/IL8 protein, human; 0/Inflammation Mediators; 0/Interleukin-8 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: SECOND-DOSE VARICELLA VACCINATION COVERAGE IN CHILDREN AND ADOLESCENTS IN A MANAGED CARE ORGANIZATIO...
Next Document: Feasibility of long-term continuous EEG-monitoring with eight electrodes the first days of life in p...