Document Detail


The Candida albicans phospholipomannan is a family of glycolipids presenting phosphoinositolmannosides with long linear chains of beta-1,2-linked mannose residues.
MedLine Citation:
PMID:  10521433     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In a series of studies, we have shown that Candida albicans synthesizes a glycolipid, phospholipomannan (PLM), which reacted with antibodies specific for beta-1,2-oligomannosides and was biosynthetically labeled by [(3)H]mannose, [(3)H]palmitic acid, and [(32)P]phosphorus. PLM has also been shown to be released from the C. albicans cell wall and to bind to and stimulate macrophage cells. In this study, we show by thin layer chromatography scanning of metabolically radiolabeled extracts that the C. albicans PLM corresponds to a family of mannose and inositol co-labeled glycolipids. We describe the purification process of the molecule and the release of its glycan fraction through alkaline hydrolysis. Analysis of this glycan fraction by radiolabeling and methylation-methanolysis confirmed the presence of inositol and of 1, 2-linked mannose units. NMR studies evidenced linear chains of beta-1,2-oligomannose as the major PLM components. Mass spectrometry analysis revealed that these chains were present in phosphoinositolmannosides with degrees of polymerization varying from 8 to 18 sugar residues. The PLM appears as a new type of eukaryotic inositol-tagged glycolipid in relationship to both the absence of glucosamine and the organization of its glycan chains. This first structural evidence for the presence of beta-1, 2-oligomannosides in a glycoconjugate other than the C. albicans phosphopeptidomannan may have some pathophysiological relevance to the adhesive, protective epitope, and signaling properties thus far established for these residues.
Authors:
P A Trinel; Y Plancke; P Gerold; T Jouault; F Delplace; R T Schwarz; G Strecker; D Poulain
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  274     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  1999 Oct 
Date Detail:
Created Date:  1999-11-23     Completed Date:  1999-11-23     Revised Date:  2000-12-18    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  30520-6     Citation Subset:  IM    
Affiliation:
Equipe Mixte de l'INSERM 99-15, Laboratoire de Mycologie Fondamentale et Appliquée, Faculté de Médecine, Centre Hospitalier Universitaire, Place de Verdun, 59045 Lille Cedex, France.
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MeSH Terms
Descriptor/Qualifier:
Candida albicans / metabolism*
Carbohydrate Sequence
Chromatography, Ion Exchange
Chromatography, Thin Layer
Glycolipids / analysis*,  biosynthesis,  chemistry*,  isolation & purification
Indicators and Reagents
Inositol / analysis
Mannose / metabolism
Molecular Sequence Data
Palmitic Acid / metabolism
Phosphorus / metabolism
Phosphorus Radioisotopes
Radioisotope Dilution Technique
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Tritium
Chemical
Reg. No./Substance:
0/Glycolipids; 0/Indicators and Reagents; 0/Phosphorus Radioisotopes; 0/phospholipomannan; 10028-17-8/Tritium; 31103-86-3/Mannose; 57-10-3/Palmitic Acid; 6917-35-7/Inositol; 7723-14-0/Phosphorus

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