Document Detail

Candida albicans and Streptococcus salivarius modulate IL-6, IL-8, and TNF-alpha expression and secretion by engineered human oral mucosa cells.
MedLine Citation:
PMID:  15469436     Owner:  NLM     Status:  MEDLINE    
We investigated the involvement of oral epithelial cells via two cytokines (IL-6 and TNF-alpha) and one chemokine (IL-8) in local defences against live yeast (Candida albicans) and bacteria (Streptococcus salivarius) using an engineered human oral mucosa model. We report that the yeast changed from the blastospore to the hyphal form and induced significant tissue disorganization at later contact periods (24 and 48 h) compared to the bacteria. However, this effect did not reduce the viability or total number of epithelial cells. Gene activation analyses revealed that IL-6, IL-8 and TNF-alpha mRNA levels rose in tissues in contact with live C. albicans or S. salivarius. Gene activation was followed by an upregulation of protein secretion. IL-6 levels were higher after contact with C. albicans than with S. salivarius. IL-8 levels after contact with S. salivarius were higher than with C. albicans. Our study suggests that S. salivarius is more efficient at inducing proinflammatory mediator release than C. albicans. These results provide additional evidence for the contribution of oral epithelial cells to the inflammatory response against fungi and bacteria.
Yakout Mostefaoui; Christian Bart; Michel Frenette; Mahmoud Rouabhia
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cellular microbiology     Volume:  6     ISSN:  1462-5814     ISO Abbreviation:  Cell. Microbiol.     Publication Date:  2004 Nov 
Date Detail:
Created Date:  2004-10-07     Completed Date:  2004-12-09     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100883691     Medline TA:  Cell Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  1085-96     Citation Subset:  IM    
Groupe de recherche en écologie buccale, Faculté de médicine dentaire, Université Laval, Quebec City, Quebec, Canada G1K 7P4.
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MeSH Terms
Candida albicans / growth & development,  pathogenicity*
Cells, Cultured
Cytokines / genetics,  metabolism*
Interleukin-6 / genetics,  metabolism
Interleukin-8 / genetics,  metabolism
Mouth Mucosa / immunology*,  microbiology
Streptococcus / pathogenicity*
Tumor Necrosis Factor-alpha / genetics,  metabolism
Reg. No./Substance:
0/Cytokines; 0/Interleukin-6; 0/Interleukin-8; 0/Tumor Necrosis Factor-alpha

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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