Document Detail

Cancer stem cell traits in squamospheres derived from primary head and neck squamous cell carcinomas.
MedLine Citation:
PMID:  21167769     Owner:  NLM     Status:  MEDLINE    
A subpopulation of cancer stem cells (CSCs), but not the majority of non-tumorigenic cancer cells, in a variety of human malignancies plays a critical role in cancer cell proliferation, invasion, metastasis, and tumor recurrence post-therapies. We report the isolation of sphere-forming cells (squamospheres) from primary head and neck squamous cell carcinomas (HNSCCs), and characterization of their CSC properties. Squamospheres appeared within 2 weeks after seeding as single-dissociated cells obtained from primary HNSCC specimens in serum-free culture conditions. Real-time RT-PCR and immunocytochemistry assays revealed that a number of stem cell markers, including CK5, OCT4, SOX2, and nestin, were up-regulated in HNSCC-driven squamospheres. Fluorescence-activated cell sorting (FACS) analysis showed that squamospheres contain enriched side population cells compared to serum-induced differentiated squamosphere cells. Furthermore, HNSCC-driven squamospheres appeared to be chemoresistant to cisplatin, 5-fluorouracil (FU), paclitaxel and doxetaxel, and showed increased levels of ABCG2, one of the ATP-binding cassette (ABC) transporters. Of particular interest, in sharp contrast to subcutaneous injection of 1×10(6) differentiated squamosphere cells, as few as 100 squamosphere cells were able to give rise to tumors in nude mice. Altogether, we assert that primary HNSCC-driven squamospheres possess CSC properties, and its functional analysis may provide a novel tool for investigating the tumorigenic process of HNSCC.
Young Chang Lim; Se-Yeong Oh; Yun Yi Cha; Sung-Hak Kim; Xun Jin; Hyunggee Kim
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-12-16
Journal Detail:
Title:  Oral oncology     Volume:  47     ISSN:  1879-0593     ISO Abbreviation:  Oral Oncol.     Publication Date:  2011 Feb 
Date Detail:
Created Date:  2011-02-07     Completed Date:  2011-05-10     Revised Date:  2014-07-31    
Medline Journal Info:
Nlm Unique ID:  9709118     Medline TA:  Oral Oncol     Country:  England    
Other Details:
Languages:  eng     Pagination:  83-91     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier Ltd. All rights reserved.
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MeSH Terms
Carcinoma / metabolism,  pathology
Carcinoma, Squamous Cell
Cell Line, Tumor
Cell Proliferation
Flow Cytometry
Head and Neck Neoplasms / metabolism,  pathology
Intermediate Filament Proteins / metabolism*
Mice, Inbred BALB C
Mice, Nude
Neoplasm Proteins / metabolism
Neoplasms, Squamous Cell / metabolism,  pathology
Neoplastic Stem Cells / metabolism*,  pathology
Nerve Tissue Proteins / metabolism*
Reverse Transcriptase Polymerase Chain Reaction
Spheroids, Cellular / metabolism*,  pathology
Reg. No./Substance:
0/Intermediate Filament Proteins; 0/NES protein, human; 0/Neoplasm Proteins; 0/Nerve Tissue Proteins; 0/Nes protein, mouse; 0/Nestin; U3P01618RT/Fluorouracil

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