| Cancer stem cell-like cells from a single cell of oral squamous carcinoma cell lines. | |
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MedLine Citation:
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PMID: 21342656 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Resistance of oral squamous cell carcinomas (OSCC) to conventional chemotherapy or radiation therapy might be due to cancer stem cells (CSCs). The development of novel anticancer drugs requires a simple method for the enrichment of CSCs. CSCs can be enriched from OSCC cell lines, for example, after cultivation in serum-free cell culture medium (SFM). In our study, we analyzed four OSCC cell lines for the presence of CSCs. CSC-like cells could not be enriched with SFM. However, cell lines obtained from holoclone colonies showed CSC-like properties such as a reduced rate of cell proliferation and a reduced sensitivity to Paclitaxel in comparison to cells from the parental lineage. Moreover, these cell lines differentially expressed the CSC-marker CD133, which is also upregulated in OSCC tissues. Interestingly, CD133(+) cells in OSCC tissues expressed little to no Ki67, the cell proliferation marker that also indicates reduced drug sensitivity. Our study shows a method for the isolation of CSC-like cell lines from OSCC cell lines. These CSC-like cell lines could be new targets for the development of anticancer drugs under in vitro conditions. |
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Authors:
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O Felthaus; T Ettl; M Gosau; O Driemel; G Brockhoff; A Reck; K Zeitler; M Hautmann; T E Reichert; G Schmalz; C Morsczeck |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-2-19 |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: - ISSN: 1090-2104 ISO Abbreviation: - Publication Date: 2011 Feb |
Date Detail:
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Created Date: 2011-2-23 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2011. Published by Elsevier Inc. |
Affiliation:
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Department of Operative Dentistry and Periodontology, University of Regensburg, Germany; Department of Oral and Maxillofacial Surgery, University of Regensburg, Germany. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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