Document Detail


Cancer cell migration within 3D layer-by-layer microfabricated photocrosslinked PEG scaffolds with tunable stiffness.
MedLine Citation:
PMID:  22809641     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Our current understanding of 3-dimensional (3D) cell migration is primarily based on results from fibrous scaffolds with randomly organized internal architecture. Manipulations that change the stiffness of these 3D scaffolds often alter other matrix parameters that can modulate cell motility independently or synergistically, making observations less predictive of how cells behave when migrating in 3D. In order to decouple microstructural influences and stiffness effects, we have designed and fabricated 3D polyethylene glycol (PEG) scaffolds that permit orthogonal tuning of both elastic moduli and microstructure. Scaffolds with log-pile architectures were used to compare the 3D migration properties of normal breast epithelial cells (HMLE) and Twist-transformed cells (HMLET). Our results indicate that the nature of cell migration is significantly impacted by the ability of cells to migrate in the third dimension. 2D ECM-coated PEG substrates revealed no statistically significant difference in cell migration between HMLE and HMLET cells among substrates of different stiffness. However, when cells were allowed to move along the third dimension, substantial differences were observed for cell displacement, velocity and path straightness parameters. Furthermore, these differences were sensitive to both substrate stiffness and the presence of the Twist oncogene. Importantly, these 3D modes of migration provide insight into the potential for oncogene-transformed cells to migrate within and colonize tissues of varying stiffness.
Authors:
Pranav Soman; Jonathan A Kelber; Jin Woo Lee; Tracy N Wright; Kenneth S Vecchio; Richard L Klemke; Shaochen Chen
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2012-07-16
Journal Detail:
Title:  Biomaterials     Volume:  33     ISSN:  1878-5905     ISO Abbreviation:  Biomaterials     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-07-30     Completed Date:  2012-11-19     Revised Date:  2013-10-17    
Medline Journal Info:
Nlm Unique ID:  8100316     Medline TA:  Biomaterials     Country:  England    
Other Details:
Languages:  eng     Pagination:  7064-70     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Elsevier Ltd. All rights reserved.
Affiliation:
Department of NanoEngineering, University of California, San Diego, La Jolla, CA 92121, USA. pranavsom@gmail.com
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MeSH Terms
Descriptor/Qualifier:
Biophysics / methods
Breast / cytology
Cell Line
Cell Line, Tumor
Cell Movement
Cross-Linking Reagents / pharmacology
Elasticity
Epithelial Cells / cytology
Equipment Design
Female
Humans
Microscopy, Electron, Scanning / methods
Neoplasm Metastasis
Neoplasms / metabolism*
Polyethylene Glycols / chemistry*
Polymers / chemistry
Stress, Mechanical
Tissue Scaffolds / chemistry*
Grant Support
ID/Acronym/Agency:
CA097022/CA/NCI NIH HHS; CA129231/CA/NCI NIH HHS; GM06852/GM/NIGMS NIH HHS; R01 EB012597/EB/NIBIB NIH HHS; R01EB012597/EB/NIBIB NIH HHS
Chemical
Reg. No./Substance:
0/Cross-Linking Reagents; 0/Polyethylene Glycols; 0/Polymers
Comments/Corrections

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