| Can we better understand the intracellular behavior of DNA nanoparticles by fluorescence correlation spectroscopy? | |
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MedLine Citation:
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PMID: 17509716 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The use of non-viral gene carriers to deliver small nucleic acids like antisense oligonucleotides (ODNs) and small interfering RNA (siRNA) remains an attractive but challenging goal in antisense therapy. Indeed, different barriers need to be overcome in the delivery process before a therapeutic effect can be obtained. One promising technique which we have been evaluating to improve our understanding of the intracellular behavior of nucleic acids/carrier complexes is Fluorescence Correlation Spectroscopy (FCS). In particular, we have used FCS for studying the protection of the nucleic acids against enzymatic degradation, and the association and dissociation of the nucleic acids with their carrier, both in buffer and in living cells. In this report, we will review our experience and findings on the use of FCS for that purpose and discuss the strengths and weaknesses of this interesting technique. |
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Authors:
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K Remaut; B Lucas; K Raemdonck; K Braeckmans; J Demeester; S C De Smedt |
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Publication Detail:
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Type: Journal Article Date: 2007-04-19 |
Journal Detail:
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Title: Journal of controlled release : official journal of the Controlled Release Society Volume: 121 ISSN: 1873-4995 ISO Abbreviation: J Control Release Publication Date: 2007 Aug |
Date Detail:
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Created Date: 2007-08-03 Completed Date: 2007-10-17 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8607908 Medline TA: J Control Release Country: Netherlands |
Other Details:
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Languages: eng Pagination: 49-63 Citation Subset: IM |
Affiliation:
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Laboratory of General Biochemistry and Physical Pharmacy, Ghent University, Harelbekestraat 72, 9000 Ghent, Belgium. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Buffers Carbocyanines / metabolism Cell Line, Tumor Cell Nucleus / metabolism Cercopithecus aethiops Cytoplasm / metabolism DNA / chemistry* Dextran Sulfate / chemistry Fluorescein-5-isothiocyanate / metabolism Fluorescence Resonance Energy Transfer Fluorescent Dyes / metabolism Humans Liposomes / chemistry Models, Chemical Nanoparticles / chemistry* Oligonucleotides, Antisense / administration & dosage, chemistry, metabolism RNA, Small Interfering / administration & dosage, chemistry Ribonuclease, Pancreatic / pharmacology Spectrometry, Fluorescence / instrumentation, methods* Vero Cells |
| Chemical | |
Reg. No./Substance:
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0/Buffers; 0/Carbocyanines; 0/Fluorescent Dyes; 0/Liposomes; 0/Oligonucleotides, Antisense; 0/RNA, Small Interfering; 0/cyanine dye 5; 3326-32-7/Fluorescein-5-isothiocyanate; 9007-49-2/DNA; 9042-14-2/Dextran Sulfate; EC 3.1.27.5/Ribonuclease, Pancreatic |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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