Document Detail

Can oxypurines plasma levels classify the type of physical exercise?
MedLine Citation:
PMID:  10399420     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: A laboratory-based model able to describe muscle energy status during physical exercise and changes in myofibrillar composition in response to training would be desirable. Lactate and ammonia concentrations are not sufficient for a comprehensive knowledge of these systems. All muscle fibres, irrespective of the type, show ATP depletion and IMP accumulation following exhausting muscular exercise with quantitative differences due to the different concentrations of deaminase. We studied the plasma concentration of metabolites of oxypurine cascade to test their reliability to classify different exercises. METHODS: We studied 52 athletes, measuring plasma metabolites at the beginning and at the end of their specific field exercise (cycle pursuers, 8 cases; soccer players, 19; marathon runners, 25). K3EDTA-blood samples were assayed for plasma hypoxanthine, xanthine, and inosine, using an HPLC technique, as well as ammonia and lactate by means of enzymatic methods. RESULTS AND CONCLUSIONS: Basal oxypurines levels were not different in relation to any specific physical exercise. Post-exercise oxypurines, namely hypoxanthine, were more precise predictors of muscle energy exhaustion than strain intensity or duration. Plasma levels of hypoxanthine may be elevated also in the presence of normal xanthine and uric acid concentrations, due to an exhaustion of the enzymatic pathway, to a reduced activity of xanthine-oxidase or finally to a substrate-dependent inhibition of the process.
G P Bianchi; G Grossi; A M Bargossi; P L Fiorella; G Marchesini
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of sports medicine and physical fitness     Volume:  39     ISSN:  0022-4707     ISO Abbreviation:  J Sports Med Phys Fitness     Publication Date:  1999 Jun 
Date Detail:
Created Date:  1999-09-14     Completed Date:  1999-09-14     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0376337     Medline TA:  J Sports Med Phys Fitness     Country:  ITALY    
Other Details:
Languages:  eng     Pagination:  123-7     Citation Subset:  IM    
Division of Internal Medicine, University of Bologna, Italy.
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MeSH Terms
Ammonia / blood
Analysis of Variance
Chromatography, High Pressure Liquid
Exercise / physiology*
Hypoxanthine / blood*
Inosine / blood*
Lactic Acid / blood
Muscle Fatigue
Uric Acid / blood
Xanthine / blood*
Reg. No./Substance:
50-21-5/Lactic Acid; 58-63-9/Inosine; 68-94-0/Hypoxanthine; 69-89-6/Xanthine; 69-93-2/Uric Acid; 7664-41-7/Ammonia

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