Document Detail


Campylobacter jejuni translocation across intestinal epithelial cells is facilitated by ganglioside-like lipooligosaccharide structures.
MedLine Citation:
PMID:  22778098     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Translocation across intestinal epithelial cells is an established pathogenic feature of the zoonotic bacterial species Campylobacter jejuni. The number of C. jejuni virulence factors known to be involved in translocation is limited. In the present study, we investigated whether sialylation of C. jejuni lipooligosaccharide (LOS) structures, generating human nerve ganglioside mimics, is important for intestinal epithelial translocation. We here show that C. jejuni isolates expressing ganglioside-like LOS bound in larger numbers to the Caco-2 intestinal epithelial cells than C. jejuni isolates lacking such structures. Next, we found that ganglioside-like LOS facilitated endocytosis of bacteria into Caco-2 cells, as visualized by quantitative microscopy using the early and late endosomal markers early endosome-associated protein 1 (EEA1), Rab5, and lysosome-associated membrane protein 1 (LAMP-1). This increased endocytosis was associated with larger numbers of surviving and translocating bacteria. Next, we found that two different intestinal epithelial cell lines (Caco-2 and T84) responded with an elevated secretion of the T-cell attractant CXCL10 to infection by ganglioside-like LOS-expressing C. jejuni isolates. We conclude that C. jejuni translocation across Caco-2 cells is facilitated by ganglioside-like LOS, which is of clinical relevance since C. jejuni ganglioside-like LOS-expressing isolates are linked with severe gastroenteritis and bloody stools in C. jejuni-infected patients.
Authors:
Rogier Louwen; Edward E S Nieuwenhuis; Leonie van Marrewijk; Deborah Horst-Kreft; Lilian de Ruiter; Astrid P Heikema; Willem J B van Wamel; Jaap A Wagenaar; Hubert P Endtz; Janneke Samsom; Peter van Baarlen; Anna Akhmanova; Alex van Belkum
Related Documents :
22791608 - Isolation of hokkaido virus, genus hantavirus, using a newly established cell line deri...
21519908 - Lkb1 signaling in advancing cell differentiation.
16240508 - Effect of clinorotation on the leaf mesophyll structure and pigment content in arabidop...
22841798 - Production of japanese encephalitis virus-like particles using the baculovirus-insect c...
7857968 - Translocation of both lysosomal ldl-derived cholesterol and plasma membrane cholesterol...
11389878 - Influences of glutathione on anionic substrate efflux in tumour cells expressing the mu...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-07-09
Journal Detail:
Title:  Infection and immunity     Volume:  80     ISSN:  1098-5522     ISO Abbreviation:  Infect. Immun.     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-08-15     Completed Date:  2012-10-24     Revised Date:  2013-07-12    
Medline Journal Info:
Nlm Unique ID:  0246127     Medline TA:  Infect Immun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3307-18     Citation Subset:  IM    
Affiliation:
Department of Medical Microbiology and Infectious Diseases, Erasmus MC, Rotterdam, The Netherlands. r.louwen@erasmusmc.nl
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Bacterial Translocation*
Campylobacter jejuni / pathogenicity*
Cell Line
Chemokine CXCL10 / secretion
Endocytosis
Epithelial Cells / microbiology*
Gangliosides / metabolism*
Humans
Lipopolysaccharides / metabolism*
Microscopy, Fluorescence
Chemical
Reg. No./Substance:
0/CXCL10 protein, human; 0/Chemokine CXCL10; 0/Gangliosides; 0/Lipopolysaccharides; 0/lipid-linked oligosaccharides
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  The Toxoplasma gondii peptide AS15 elicits CD4 T cells that can control parasite burden.
Next Document:  FimA, FimF, and FimH are necessary for assembly of type 1 fimbriae on Salmonella enterica serovar Ty...