Document Detail


Campylobacter jejuni survival within human epithelial cells is enhanced by the secreted protein CiaI.
MedLine Citation:
PMID:  21435039     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Although it is known that Campylobacter jejuni invade the cells that line the human intestinal tract, the bacterial proteins that enable this pathogen to survive within Campylobacter-containing vacuoles (CCV) have not been identified. Here, we describe the identification and characterization of a protein that we termed CiaI for Campylobacter invasion antigen involved in intracellular survival. We show that CiaI harbours an amino-terminal type III secretion sequence and is secreted from C. jejuni through the flagellar type III secretion system. In addition, the ciaI mutant was impaired in intracellular survival when compared with a wild-type strain, as judged by the gentamicin-protection assay. Fluorescence microscopy examination of epithelial cells infected with the C. jejuni ciaI mutant revealed that the CCV were more frequently co-localized with Cathepsin D (a lysosomal marker) than the CCV in cells infected with a C. jejuni wild-type strain. Ectopic expression of CiaI-GFP in epithelial cells yielded a punctate phenotype not observed with the other C. jejuni genes, and this phenotype was abolished by mutation of a dileucine motif located in the carboxy-terminus of the protein. Based on the data, we conclude that CiaI contributes to the ability of C. jejuni to survive within epithelial cells.
Authors:
Daelynn R Buelow; Jeffrey E Christensen; Jason M Neal-McKinney; Michael E Konkel
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2011-04-11
Journal Detail:
Title:  Molecular microbiology     Volume:  80     ISSN:  1365-2958     ISO Abbreviation:  Mol. Microbiol.     Publication Date:  2011 Jun 
Date Detail:
Created Date:  2011-05-27     Completed Date:  2011-09-21     Revised Date:  2013-06-30    
Medline Journal Info:
Nlm Unique ID:  8712028     Medline TA:  Mol Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  1296-312     Citation Subset:  IM    
Copyright Information:
© 2011 Blackwell Publishing Ltd.
Affiliation:
School of Molecular Biosciences, College of Veterinary Medicine, Washington State University, Pullman, Washington 99164-7520, USA.
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MeSH Terms
Descriptor/Qualifier:
Bacterial Proteins / genetics,  metabolism*
Campylobacter Infections / microbiology*
Campylobacter jejuni / genetics,  growth & development*,  metabolism*
Epithelial Cells / microbiology*
Gene Expression Regulation, Bacterial
Humans
Microbial Viability*
Protein Transport
Grant Support
ID/Acronym/Agency:
N01 AI030055/AI/NIAID NIH HHS; N01-AL-30055//PHS HHS; T32 GM083864-03/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Bacterial Proteins
Comments/Corrections

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