Document Detail


Camelliin B induced apoptosis in HeLa cell line.
MedLine Citation:
PMID:  11684320     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Gordonia axillaris (Roxb.) Dietrich (Theaceae) is a native to Taiwan and the leaves have been used as an astringent folk medicine. Camelliin B (CB), a macrocyclic hydrolyzable tannin, was isolated from G. axillaris and showed cytotoxic effects in human carcinoma cells. Among the target cells (SKHep-1, Ha-22T, DU-145, AGS, and HeLa), the cervical carcinoma cell line, HeLa, was more sensitive to CB than were Chang normal liver cells and primary-cultured normal gingival and cervical fibroblasts. Furthermore, the cytotoxic effects of CB showed dose-dependency at 3.2-100.0 microg/ml in HeLa for 1,24,48, and 72 h and with an IC(50) value of 46.3 microg/ml for 48 h. However, the IC(50) value of CB in primary-cultured normal cervical fibroblasts was 108.0 microg/ml. Therefore, the selectivity shown by CB was ascribed to the difference in growth speed between normal and tumor cells. HeLa cells and primary-cultured normal cervical fibroblasts were treated with 50.0 and 100.0 microg/ml CB for 48 h, respectively, and exhibited chromatin condensation, indicating the occurrence of apoptosis. Flow cytometric analysis demonstrated the presence of apoptotic cells with low DNA content, a decrease of cell population at the G(1) phase, and a concomitant increase of cell population at the G(2)/M phase. CB also caused DNA fragmentation and inhibited PARP degradation in HeLa cells. However, CB did not significantly inhibit Bcl-2 expression in HeLa cells at 50.0 microg/ml, only at 100.0 microg/ml for 48 h. These results suggest that CB induced apoptosis, without direct inhibition of Bcl-2 expression in HeLa cells.
Authors:
C C Wang; L G Chen; L L Yang
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Toxicology     Volume:  168     ISSN:  0300-483X     ISO Abbreviation:  Toxicology     Publication Date:  2001 Nov 
Date Detail:
Created Date:  2001-10-30     Completed Date:  2001-12-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0361055     Medline TA:  Toxicology     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  231-40     Citation Subset:  IM    
Affiliation:
Graduate Institute of Pharmacognosy Science, Taipei Medical University, 250 Wu-Hsing Street, Taipei 110, Taiwan, ROC.
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MeSH Terms
Descriptor/Qualifier:
Antineoplastic Agents, Phytogenic / chemistry,  toxicity*
Apoptosis / drug effects*
Blotting, Western
DNA Fragmentation / drug effects
Electrophoresis, Agar Gel
Fibroblasts / drug effects
Flow Cytometry
Hela Cells
Humans
Hydrolyzable Tannins*
Plants, Medicinal / chemistry*
Poly(ADP-ribose) Polymerases / metabolism
Taiwan
Tannins / chemistry,  toxicity*
Theaceae / chemistry*
Tumor Cells, Cultured
Chemical
Reg. No./Substance:
0/Antineoplastic Agents, Phytogenic; 0/Hydrolyzable Tannins; 0/Tannins; 126347-60-2/camelliin B; EC 2.4.2.30/Poly(ADP-ribose) Polymerases

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