| Camel urine components display anti-cancer properties in vitro. | |
| | |
MedLine Citation:
|
PMID: 22922085 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
AIM OF THE STUDY: While camel urine (CU) is widely used in the Arabian Peninsula to treat various diseases, including cancer, its exact mechanism of action is still not defined. The objective of the present study is to investigate whether camel urine has anti-cancer effect on human cells in vitro. MATERIALS AND METHODS: The annexinV/PI assay was used to assess apoptosis, and immunoblotting analysis determined the effect of CU on different apoptotic and oncogenic proteins. Furthermore, flow cytometry and Elispot were utilized to investigate cytotoxicity and the effect on the cell cycle as well as the production of cytokines, respectively. RESULTS: Camel urine showed cytotoxicity against various, but not all, human cancer cell lines, with only marginal effect on normal epithelial and fibroblast cells. Interestingly, 20mg/ml of lyophilized CU inhibited cell proliferation and triggered more than 80% of apoptosis in different cancer cells, including breast carcinomas and medulloblastomas. Apoptosis was induced in these cells through the intrinsic pathway via Bcl-2 decrease. Furthermore, CU down-regulated the cancer-promoting proteins survivin, β-catenin and cyclin D1 and increased the level of the cyclin-dependent kinase inhibitor p21. In addition, we have shown that CU has no cytotoxic effect against peripheral blood mononuclear cells and has strong immuno-inducer activity through inducing IFN-γ and inhibiting the Th2 cytokines IL-4, IL-6 and IL-10. CONCLUSIONS: CU has specific and efficient anti-cancer and potent immune-modulator properties in vitro. |
| | |
Authors:
|
Nujoud Al-Yussef; Ameera Gaafar; Basem Al-Otaibi; Ibrahim Al-Jammaz; Khaled Al-Hussein; Abdelilah Aboussekhra |
Related Documents
:
|
23311665 - Redox control of inflammation in macrophages. 23213355 - Disparate roles of marrow- and parenchymal cell-derived tlr4 signaling in murine lps-in... 23347745 - Improvement of porcine cloning efficiency by trichostain a through early-stage inductio... 9837855 - Glutathione is implied in the control of 7-ketocholesterol-induced apoptosis, which is ... 2677735 - Immune response in mice infected with candida albicans in the mycelial form. 14686485 - T-cell control by human t-cell leukemia/lymphoma virus type 1. |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2012-8-16 |
Journal Detail:
|
Title: Journal of ethnopharmacology Volume: - ISSN: 1872-7573 ISO Abbreviation: J Ethnopharmacol Publication Date: 2012 Aug |
Date Detail:
|
Created Date: 2012-8-27 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 7903310 Medline TA: J Ethnopharmacol Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
|
Copyright © 2012 Elsevier Ireland Ltd. All rights reserved. |
Affiliation:
|
Department of Biological and Medical Research, King Faisal Specialist Hospital and Research Center, MBC # 03, PO BOX 3354, Riyadh 11211, Saudi Arabia. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Effects of food ingredients and oxygen exposure on premature browning in cooked beef.
Next Document: The roots of Salvia miltiorrhiza (Danshen) and Pueraria lobata (Gegen) inhibit atherogenic events: A...