| Calpains and cytokines in fibrillating human atria. | |
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MedLine Citation:
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PMID: 12063299 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Atrial fibrillation (AF) is accompanied by intracellular calcium overload. The purpose of this study was to assess the role of calcium-dependent calpains and cytokines during AF. Atrial tissue samples from 32 patients [16 with chronic AF and 16 in sinus rhythm (SR)] undergoing open heart surgery were studied. Atrial expression of calpain I and II, calpastatin, troponin T (TnT), troponin C (TnC), and cytokines [interleukin (IL)-1 beta, IL-2, IL-6, IL-8, IL-10, transforming growth factor (TGF)-beta 1, and tumor necrosis factor-alpha] were determined. Expression of calpain I was increased during AF (461 +/- 201% vs. 100 +/- 34%, P < 0.05). Amounts of calpain II and calpastatin were unchanged. Total calpain enzymatic activity was more than doubled during AF (35.2 +/- 17.7 vs. 12.4 +/- 9.2 units, P < 0.05). In contrast to TnC, TnT levels were reduced in fibrillating atria by 26% (P < 0.05), corresponding to the myofilament disintegration seen by electron microscopy. Small amounts of only IL-2 and TGF-beta 1 mRNA and protein were detected regardless of the underlying cardiac rhythm. In conclusion, atria of patients with permanent AF show evidence of calpain I activation that might contribute to structural remodeling and contractile dysfunction, whereas there is no evidence of activation of tissue cytokines. |
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Authors:
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Andreas Goette; Marco Arndt; Christoph Röcken; Thorsten Staack; Roland Bechtloff; Dirk Reinhold; Christof Huth; Siegfried Ansorge; Helmut U Klein; Uwe Lendeckel |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: American journal of physiology. Heart and circulatory physiology Volume: 283 ISSN: 0363-6135 ISO Abbreviation: Am. J. Physiol. Heart Circ. Physiol. Publication Date: 2002 Jul |
Date Detail:
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Created Date: 2002-06-13 Completed Date: 2002-07-16 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 100901228 Medline TA: Am J Physiol Heart Circ Physiol Country: United States |
Other Details:
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Languages: eng Pagination: H264-72 Citation Subset: IM |
Affiliation:
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Division of Cardiology, Department of Internal Medicine, University Hospital Magdeburg, 39120 Magdeburg, Germany. andreas.goette@medizin.uni-magdeburg.de |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Aged Atrial Appendage / physiopathology*, ultrastructure Atrial Fibrillation / pathology, physiopathology* Blotting, Western Calcium-Binding Proteins / biosynthesis*, genetics Calpain / biosynthesis*, genetics Cytokines / biosynthesis*, genetics Female Humans Interleukin-2 / biosynthesis, genetics Male Microfilaments / pathology, ultrastructure Middle Aged Myocardium / metabolism, pathology, ultrastructure RNA, Messenger / metabolism Transforming Growth Factor beta / biosynthesis, genetics Transforming Growth Factor beta1 |
| Chemical | |
Reg. No./Substance:
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0/Calcium-Binding Proteins; 0/Cytokines; 0/Interleukin-2; 0/RNA, Messenger; 0/TGFB1 protein, human; 0/Transforming Growth Factor beta; 0/Transforming Growth Factor beta1; 79079-11-1/calpastatin; EC 3.4.22.-/Calpain |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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