Document Detail

Calpain inhibitor-1 protects the rat heart from ischemia-reperfusion injury: analysis by mechanical work and energetics.
MedLine Citation:
PMID:  15528229     Owner:  NLM     Status:  MEDLINE    
We hypothesized that calpain inhibitor-1 protected left ventricular (LV) function from ischemia-reperfusion injury by inhibiting the proteolysis of alpha-fodrin. To test this hypothesis, we investigated the effect of calpain inhibitor-1 on LV mechanical work and energetics in the cross-circulated rat hearts that underwent 15-min global ischemia and 60-min reperfusion (n = 9). After ischemia-reperfusion with calpain inhibitor-1, mean end-systolic pressure at midrange LV volume and systolic pressure-volume area (PVA) at midrange LV volume (total mechanical energy per beat) were hardly changed, although they were significantly (P < 0.01) decreased after ischemia-reperfusion without calpain inhibitor-1. Mean myocardial oxygen consumption per beat (Vo(2)) intercepts (PVA-independent Vo(2); Vo(2) for the total Ca(2+) handling in excitation-contraction coupling and basal metabolism) of Vo(2)-PVA linear relations were also unchanged after ischemia-reperfusion with calpain inhibitor-1, although they were significantly (P < 0.01) decreased after ischemia-reperfusion without calpain inhibitor-1. There were no significant differences in O(2) costs of LV PVA and contractility among the hearts in control (or normal) postischemia-reperfusion and postischemia-reperfusion with calpain inhibitor-1. Western blot analysis of alpha-fodrin and the immunostaining of 150-kDa products of alpha-fodrin confirmed that calpain inhibitor-1 almost completely protected the proteolysis of alpha-fodrin. Our results indicate that calpain inhibitor-1 prevents the heart from ischemia-reperfusion injury associated with the impairment of total Ca(2+) handling by directly inhibiting the proteolysis of alpha-fodrin.
Yoshiro Yoshikawa; Hiroji Hagihara; Yoshimi Ohga; Chikako Nakajima-Takenaka; Ken-ya Murata; Shigeki Taniguchi; Miyako Takaki
Related Documents :
8587439 - Cardiac tissue endothelin-1 levels under basal, stimulated, and ischemic conditions.
9695119 - Midazolam is a safe agent by comparison with thiopentone on arrhythmias in ischaemia an...
8369469 - Postcardioplegia acute cardiac dysfunction and reperfusion injury.
2031929 - Twelve-hour cardiopulmonary preservation using donor core cooling, leukocyte depletion,...
7223899 - Intracellular recording of in situ ventricular cells during ventricular fibrillation.
18437149 - Effect of pressure overload on cardioprotection of mitochondrial katp channels and gsk-...
1315649 - Oestradiol-induced hypotension in spontaneously hypertensive rats: putative role for in...
12973419 - The potential use of gene therapy in the control of hypertension.
9779139 - Bilateral icp monitoring: its importance in detecting the severity of secondary insults.
Publication Detail:
Type:  Journal Article     Date:  2004-11-04
Journal Detail:
Title:  American journal of physiology. Heart and circulatory physiology     Volume:  288     ISSN:  0363-6135     ISO Abbreviation:  Am. J. Physiol. Heart Circ. Physiol.     Publication Date:  2005 Apr 
Date Detail:
Created Date:  2005-03-17     Completed Date:  2005-04-20     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  100901228     Medline TA:  Am J Physiol Heart Circ Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  H1690-8     Citation Subset:  IM    
Dept. of Physiology II, Nara Medical University, 840 Shijo-cho, Kashihara, Nara 634-8521, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Blood Pressure / drug effects,  physiology
Blotting, Western
Calcium / metabolism
Calcium-Transporting ATPases / metabolism
Cardiotonic Agents / pharmacology
Carrier Proteins / metabolism
Cysteine Proteinase Inhibitors / pharmacology*
Electrophoresis, Polyacrylamide Gel
Energy Metabolism / drug effects,  physiology
Glycoproteins / pharmacology*
Lactic Acid / metabolism
Microfilament Proteins / metabolism
Myocardial Contraction / drug effects,  physiology
Myocardial Reperfusion Injury / drug therapy*,  physiopathology*
Oxygen Consumption / drug effects,  physiology
Rats, Wistar
Sarcoplasmic Reticulum Calcium-Transporting ATPases
Sodium-Calcium Exchanger / metabolism
Sodium-Potassium-Exchanging ATPase / metabolism
Ventricular Function, Left / drug effects,  physiology
Reg. No./Substance:
0/Cardiotonic Agents; 0/Carrier Proteins; 0/Cysteine Proteinase Inhibitors; 0/Glycoproteins; 0/Microfilament Proteins; 0/Sodium-Calcium Exchanger; 0/calpain inhibitors; 0/fodrin; 50-21-5/Lactic Acid; 7440-70-2/Calcium; EC ATPases; EC Reticulum Calcium-Transporting ATPases; EC ATPase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Regulation of actin dynamics is critical for endothelial barrier functions.
Next Document:  Molecular and phenotypic effects of heterozygous, homozygous, and compound heterozygote myosin heavy...