| Calpain inhibition stimulates caspase-dependent apoptosis induced by taxol in NIH3T3 cells. | |
| | |
MedLine Citation:
|
PMID: 17145055 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Taxol is an anticancer drug that triggers apoptosis in a wide spectrum of cancers such as ovarian, breast, lung, head and neck, and bladder carcinoma by both caspase-dependent and -independent apoptosis mechanisms. However, the exact signaling pathways involved in taxol-induced apoptosis strongly depend on the cellular background and they are not completely established yet. In this study we demonstrate that taxol induces caspase-3-independent apoptosis in NIH3T3 cells by a calpain-mediated mechanism. Taxol treatment produced changes in the mitochondrial membrane potential (Delta Psi m) which could be responsible of Ca(2+) release from the mitochondria and the consequent calpain activation. Interestingly, we show that calpain produced proteolysis of caspase-3 and demonstrate that, accordingly, calpain inhibition increased taxol-induced apoptosis. In addition, we reveal that poly (ADP-ribose) polymerase (PARP) was processed by calpain in taxol-treated cells and by caspase-3 after calpain inhibition. In conclusion, these results demonstrate for the first time that calpain could play an important role modulating taxol-induced apoptosis. Further studies are needed to address the potentiality of inducing apoptosis by a combined use of taxol and calpain inhibitors in cells with increased calpain activity. |
| | |
Authors:
|
David Piñeiro; M Elena Martín; Natalia Guerra; Matilde Salinas; Víctor M González |
Related Documents
:
|
19191105 - Nuclear localization of active matrix metalloproteinase-2 in cigarette smoke-exposed ap... 21335935 - Frequency alterations in key innate immune cell components in the peripheral blood of d... 16170665 - Invasion-associated mmp-2 and mmp-9 are up-regulated intracellularly in concert with ap... 15075075 - Use of a novel histone deacetylase inhibitor to induce apoptosis in cell lines of acute... 20440285 - Cytokines as therapeutic targets in sle. 11787815 - Apoptotic proteins. p53 and c-myc related pathways. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-10-26 |
Journal Detail:
|
Title: Experimental cell research Volume: 313 ISSN: 0014-4827 ISO Abbreviation: Exp. Cell Res. Publication Date: 2007 Jan |
Date Detail:
|
Created Date: 2007-01-12 Completed Date: 2007-03-09 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0373226 Medline TA: Exp Cell Res Country: United States |
Other Details:
|
Languages: eng Pagination: 369-79 Citation Subset: IM |
Affiliation:
|
Servicio de Bioquímica-Investigación, Hospital Ramón y Cajal, Ctra Colmenar km 9,100, 28034 Madrid, Spain. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Antineoplastic Agents, Phytogenic / pharmacology* Apoptosis* / drug effects Calcium / analysis, metabolism Calpain / antagonists & inhibitors, physiology* Caspase 3 / antagonists & inhibitors, physiology* Cysteine Proteinase Inhibitors / pharmacology Cytoplasm / chemistry, metabolism Dipeptides / pharmacology Membrane Potential, Mitochondrial / drug effects Mice NIH 3T3 Cells Paclitaxel / pharmacology* |
| Chemical | |
Reg. No./Substance:
|
0/Antineoplastic Agents, Phytogenic; 0/Cysteine Proteinase Inhibitors; 0/Dipeptides; 33069-62-4/Paclitaxel; 7440-70-2/Calcium; 88191-84-8/calpain inhibitor III; EC 3.4.22.-/Calpain; EC 3.4.22.-/Caspase 3 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: A study on fuzzy C-means clustering-based systems in automatic spike detection.
Next Document: The xenoestrogen, 4-nonylphenol, impaired steroidogenesis in previtellogenic oocyte culture of Atlan...