Document Detail


Calpain-3 is autolyzed and hence activated in human skeletal muscle 24 h following a single bout of eccentric exercise.
MedLine Citation:
PMID:  17585039     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The function and normal regulation of calpain-3, a muscle-specific Ca(2+)-dependent protease, is uncertain, although its absence leads to limb-girdle muscular dystrophy type 2A. This study examined the effect of eccentric exercise on calpain-3 autolytic activation, because such exercise is known to damage sarcomeric structures and to trigger adaptive changes that help prevent such damage on subsequent exercise. Six healthy human subjects performed a 30-min bout of one-legged, eccentric, knee extensor exercise. Torque measurements, vastus lateralis muscle biopsies, and venous blood samples were taken before and up to 7 days following the exercise. Peak isometric muscle torque was depressed immediately and at 3 h postexercise and recovered by 24 h, and serum creatine kinase concentration peaked at 24 h postexercise. The amount of autolyzed calpain-3 was unchanged immediately and 3 h after exercise, but increased markedly (from approximately 16% to approximately 35% of total) 24 h after the exercise, and returned to preexercise levels within 7 days. In contrast, the eccentric exercise produced little autolytic activation of the ubiquitous Ca(2+)-activated protease, mu-calpain. Eccentric exercise is the first physiological circumstance shown to result in calpain-3 activation in vivo.
Authors:
Robyn M Murphy; Craig A Goodman; Michael J McKenna; Jason Bennie; Murray Leikis; Graham D Lamb
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-06-21
Journal Detail:
Title:  Journal of applied physiology (Bethesda, Md. : 1985)     Volume:  103     ISSN:  8750-7587     ISO Abbreviation:  J. Appl. Physiol.     Publication Date:  2007 Sep 
Date Detail:
Created Date:  2007-08-28     Completed Date:  2007-12-20     Revised Date:  2013-09-26    
Medline Journal Info:
Nlm Unique ID:  8502536     Medline TA:  J Appl Physiol (1985)     Country:  United States    
Other Details:
Languages:  eng     Pagination:  926-31     Citation Subset:  IM    
Affiliation:
Department of Zoology, La Trobe University, Victoria 3086, Australia. r.murphy@latrobe.edu.au
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Calpain / metabolism*
Creatine Kinase / blood
Exercise / physiology*
Female
Humans
Male
Muscle Proteins / metabolism*
Quadriceps Muscle / metabolism*
Time Factors
Torque
Chemical
Reg. No./Substance:
0/Muscle Proteins; EC 2.7.3.2/Creatine Kinase; EC 3.4.22.-/CAPN3 protein, human; EC 3.4.22.-/Calpain; EC 3.4.22.-/mu-calpain

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