Document Detail

Calorimetric control for high cell density cultivation of a recombinant Escherichia coli strain.
MedLine Citation:
PMID:  20083146     Owner:  NLM     Status:  MEDLINE    
In order to achieve maximum productivity of recombinant proteins in Escherichia coli high cell density cultivation (HCDC) strategies have been the subject of many studies. The aim of this work was the application of calorimetric methods to HCDC. The specific growth rate of a recombinant E. coli strain producing green fluorescent protein (GFP) was controlled during fed-batch cultivations by estimating the specific growth rate from the measured heat flow produced by the cells. For the cultivation a standard 30 l laboratory bioreactor was used, which was extended in such a way that heat balancing is possible. The feed rate was adjusted by an adaptive controller such that the specific growth rate was kept on the desired set point value. On the basis of experimental investigations with a recombinant E. coli strain using glucose as limiting C-source it was demonstrated that the specific growth rate can be kept on a given set point value and biomass concentrations of up to 120 g l(-1) can be obtained, reproducibly.
Richard Biener; Anne Steink?mper; Johannes Hofmann
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Publication Detail:
Type:  Journal Article     Date:  2010-01-18
Journal Detail:
Title:  Journal of biotechnology     Volume:  146     ISSN:  1873-4863     ISO Abbreviation:  J. Biotechnol.     Publication Date:  2010 Mar 
Date Detail:
Created Date:  2010-03-03     Completed Date:  2010-05-11     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8411927     Medline TA:  J Biotechnol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  45-53     Citation Subset:  IM    
Copyright Information:
Copyright 2010 Elsevier B.V. All rights reserved.
University of Applied Sciences Esslingen, Department of Natural Sciences, 73728 Esslingen, Germany.
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MeSH Terms
Bacteriological Techniques / methods*
Calorimetry / methods*
Escherichia coli / growth & development*,  metabolism
Glucose / metabolism
Oxygen / metabolism
Recombinant Proteins / biosynthesis*
Reg. No./Substance:
0/Recombinant Proteins; 50-99-7/Glucose; 7782-44-7/Oxygen

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