| Caloric restriction increases free radicals and inducible nitric oxide synthase expression in mice infected with Salmonella Typhimurium. | |
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MedLine Citation:
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PMID: 20883207 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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It is well known that CR (caloric restriction) reduces oxidative damage to proteins, lipids and DNA, although the underlying mechanism is unclear. However, information concerning the effect of CR on the host response to infection is sparse. In this study, 6-month-old mice that were fed AL (ad libitum) or with a CR diet were infected with Salmonella serovar Typhimurium. EPR (electron paramagnetic resonance; also known as ESR (electron spin resonance)) was used to identify FRs (free radicals). These results were subsequently correlated with SOD (superoxide dismutase) catalytic activity, iNOS [inducible NOS (nitric oxide synthase) or NOSII] expression and NO (nitric oxide) content. EPR analysis of liver samples demonstrated that there was a higher quantity of FRs and iron-nitrosyl complex in infected mice provided with a CR diet as compared with those on an AL diet, indicating that CR was beneficial by increasing the host response to Salmonella Typhimurium. Furthermore, in infected mice on the CR diet, NOSII expression was higher, NO content was greater and spleen colonization was lower, compared with mice on the AL diet. No changes in SOD activity were detected, indicating that the NO produced participated more in the formation of iron-nitrosyl complexes than peroxynitrite. These results suggest that CR exerts a protective effect against Salmonella Typhimurium infection by increasing NO production. |
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Authors:
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José Trujillo-Ferrara; Rafael Campos-Rodríguez; Eleazar Lara-Padilla; Daniel Ramírez-Rosales; José Correa Basurto; Angel Miliar Garcia; Humberto Reyna Garfias; Rafael Zamorano Ulloa; Martha C Rosales-Hernández |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Bioscience reports Volume: 31 ISSN: 1573-4935 ISO Abbreviation: Biosci. Rep. Publication Date: 2011 Aug |
Date Detail:
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Created Date: 2011-03-03 Completed Date: 2011-07-12 Revised Date: 2011-10-24 |
Medline Journal Info:
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Nlm Unique ID: 8102797 Medline TA: Biosci Rep Country: United States |
Other Details:
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Languages: eng Pagination: 273-82 Citation Subset: IM |
Affiliation:
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Sección de Estudios de Posgrado e Investigación y Departamento de Bioquímica de la Escuela Superior de Medicina del Instituto Politécnico Nacional, Plan de San Luis y Díaz Mirón s/n, D.F. 11340, Mexico. |
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Caloric Restriction* Free Radicals / metabolism* Male Mice Mice, Inbred BALB C Nitric Oxide / metabolism Nitric Oxide Synthase Type II / metabolism* Salmonella Infections / enzymology*, metabolism Salmonella typhimurium* Superoxide Dismutase / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Free Radicals; 10102-43-9/Nitric Oxide; EC 1.14.13.39/Nitric Oxide Synthase Type II; EC 1.15.1.1/Superoxide Dismutase |
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