Document Detail

Calmodulin and CaMKII in the sperm principal piece: evidence for a motility-related calcium/calmodulin pathway.
MedLine Citation:
PMID:  17460096     Owner:  NLM     Status:  MEDLINE    
Both cyclic AMP (cAMP)/protein kinase A (PKA) and calcium (Ca(2+)) signaling pathways are known to be involved in the regulation of motility in mammalian sperm. Calmodulin (CaM) is a ubiquitous Ca(2+) sensor that has been implicated in the acrosome reaction. In this report, we identify an insoluble pool of CaM in sperm and show that the protein, in addition to its presence in the acrosome, is found in the principal piece of the flagellum. These findings are consistent with, though not proof of, the presence of a pool of CaM in the fibrous sheath. The Ca(2+)/CaM-dependent protein kinase IIbeta (CaMKIIbeta), which is a downstream target of Ca(2+)/CaM, similarly localizes to the principal piece. In addition, we confirm earlier reports that a CaM inhibitor decreases sperm motility. However, we find that this inhibition can be largely reversed by stimulation of PKA if substrates for oxidative respiration are present in the medium. Our results suggest that the Ca(2+)/CaM/CaMKII signaling pathway in the sperm principal piece is involved in regulating sperm motility, and that this pathway functions either in parallel with or upstream of the cAMP/PKA pathway.
Karen Schlingmann; Marcela A Michaut; John L McElwee; Collin A Wolff; Alexander J Travis; Regina M Turner
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2007-04-25
Journal Detail:
Title:  Journal of andrology     Volume:  28     ISSN:  0196-3635     ISO Abbreviation:  J. Androl.     Publication Date:    2007 Sep-Oct
Date Detail:
Created Date:  2007-08-22     Completed Date:  2007-12-27     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8106453     Medline TA:  J Androl     Country:  United States    
Other Details:
Languages:  eng     Pagination:  706-16     Citation Subset:  IM    
New Bolton Center, University of Pennsylvania School of Veterinary Medicine, 382 West Street Rd, Kennett Square, PA 19348, USA.
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MeSH Terms
A Kinase Anchor Proteins / metabolism
Calcium-Calmodulin-Dependent Protein Kinase Type 2 / metabolism*
Calmodulin / antagonists & inhibitors,  metabolism*
Cyclic AMP-Dependent Protein Kinases / metabolism
Cytoskeleton / metabolism
Fluorescent Antibody Technique, Indirect
Lactic Acid / metabolism
Pyruvic Acid / metabolism
Signal Transduction / physiology*
Sperm Motility / physiology*
Sperm Tail / enzymology,  metabolism*
Grant Support
Reg. No./Substance:
0/A Kinase Anchor Proteins; 0/Akap4 protein, mouse; 0/Calmodulin; 127-17-3/Pyruvic Acid; 50-21-5/Lactic Acid; EC AMP-Dependent Protein Kinases; EC Protein Kinase Type 2

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