| Calibration method for small animal indirect calorimeters. | |
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MedLine Citation:
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PMID: 1951693 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A simple mini-butane burner or "artificial rat," for calibration of small animal indirect calorimeters (modified Benedict and Haldane systems), was developed. Substitution of this burner for a live animal at regular intervals in an experimental protocol provides a means by which whole system function can be verified without disturbing respirometer conditions. Heat production, degree of total combustion of butane, proportion of unburned butane, and the CO2 production (VCO2)-to-O2 consumption (VO2) ratio were determined in animal and burner experiments by direct measurement or were derived from mass equations. Burner security was also discussed. VCO2/VO2 obtained in calibration experiments using the Benedict system remained constant and was not significantly different from the expected value for total combustion of butane. Observation of a stabilization phase in the Haldane burner experiments illustrated the utility of calibration over the duration of a normal animal experiment. This miniburner gave highly reproducible results and simulated the daily VO2, VCO2, and heat production of an adult rat. |
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Authors:
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S J MacKay; A Loiseau; R Poivre; A Huot |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The American journal of physiology Volume: 261 ISSN: 0002-9513 ISO Abbreviation: Am. J. Physiol. Publication Date: 1991 Nov |
Date Detail:
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Created Date: 1991-12-20 Completed Date: 1991-12-20 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0370511 Medline TA: Am J Physiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: E661-4 Citation Subset: IM |
Affiliation:
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Institut National de la Santé et de la Recherche Médicale (INSERM) Unité 1, Hôpital Bichat, Paris, France. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Calibration Calorimetry, Indirect / instrumentation*, methods Carbon Dioxide / metabolism Equipment Design Oxygen Consumption Temperature |
| Chemical | |
Reg. No./Substance:
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124-38-9/Carbon Dioxide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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