| Calf production from vitrified bovine sexed embryos following in-straw dilution. | |
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MedLine Citation:
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PMID: 20662815 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The objective of this study was to develop an in-straw dilution method suitable for direct transfer of vitrified bovine sexed embryos. Embryo sexing was performed by molecular diagnosis. Several sexed and vitrified-warmed embryos were transferred after evaluation of morphologically embryonic survival at warming and in-straw dilution (Evaluation group). The other embryos were immediately directly transferred to recipients without first being expelled from the straws after in-straw dilution (Non-evaluation group). The pregnancy rates of vitrified sexed embryos were 38.7% and 34.8% in the Evaluation group and Non-evaluation group, respectively, which were not significantly different. The viability of lower quality embryos before vitrification tended to be lower (P = 0.087) than that of the higher quality embryos regardless of evaluating embryos after warming and in-straw dilution. The abortion rates were similar, and there was no difference between the two groups (13.9% and 12.5%, respectively). These results demonstrate that vitrified bovine sexed embryos can be vitrified and diluted by the in-straw method and that the vitrified and warmed sexed embryos can develop to term. |
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Authors:
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Kiyoshi Akiyama; Jun Kobayashi; Yoshimasa Sato; Ryuichi Sata; Megumi Ohashi; Emi Sasaki; Yorimasa Oda; Yoshio Ogawa; Shuji Ueda; Hisashi Nabenishi; Satoko Matoba |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Animal science journal = Nihon chikusan Gakkaihō Volume: 81 ISSN: 1740-0929 ISO Abbreviation: Anim. Sci. J. Publication Date: 2010 Aug |
Date Detail:
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Created Date: 2010-07-28 Completed Date: 2010-12-30 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100956805 Medline TA: Anim Sci J Country: Australia |
Other Details:
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Languages: eng Pagination: 461-6 Citation Subset: IM |
Affiliation:
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Kanagawa Prefectural Livestock Industry Technical Center, Ebina, Kanagawa. akiyama.xqnm@pref.kanagawa.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cattle / physiology* Embryo Transfer / methods* Sex Determination Analysis* Vitrification* |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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