Document Detail

Calf production from vitrified bovine sexed embryos following in-straw dilution.
MedLine Citation:
PMID:  20662815     Owner:  NLM     Status:  MEDLINE    
The objective of this study was to develop an in-straw dilution method suitable for direct transfer of vitrified bovine sexed embryos. Embryo sexing was performed by molecular diagnosis. Several sexed and vitrified-warmed embryos were transferred after evaluation of morphologically embryonic survival at warming and in-straw dilution (Evaluation group). The other embryos were immediately directly transferred to recipients without first being expelled from the straws after in-straw dilution (Non-evaluation group). The pregnancy rates of vitrified sexed embryos were 38.7% and 34.8% in the Evaluation group and Non-evaluation group, respectively, which were not significantly different. The viability of lower quality embryos before vitrification tended to be lower (P = 0.087) than that of the higher quality embryos regardless of evaluating embryos after warming and in-straw dilution. The abortion rates were similar, and there was no difference between the two groups (13.9% and 12.5%, respectively). These results demonstrate that vitrified bovine sexed embryos can be vitrified and diluted by the in-straw method and that the vitrified and warmed sexed embryos can develop to term.
Kiyoshi Akiyama; Jun Kobayashi; Yoshimasa Sato; Ryuichi Sata; Megumi Ohashi; Emi Sasaki; Yorimasa Oda; Yoshio Ogawa; Shuji Ueda; Hisashi Nabenishi; Satoko Matoba
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Animal science journal = Nihon chikusan Gakkaihō     Volume:  81     ISSN:  1740-0929     ISO Abbreviation:  Anim. Sci. J.     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-07-28     Completed Date:  2010-12-30     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100956805     Medline TA:  Anim Sci J     Country:  Australia    
Other Details:
Languages:  eng     Pagination:  461-6     Citation Subset:  IM    
Kanagawa Prefectural Livestock Industry Technical Center, Ebina, Kanagawa.
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MeSH Terms
Cattle / physiology*
Embryo Transfer / methods*
Sex Determination Analysis*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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