Document Detail


Caldicellulosiruptor obsidiansis sp. nov., an anaerobic, extremely thermophilic, cellulolytic bacterium isolated from Obsidian Pool, Yellowstone National Park.
MedLine Citation:
PMID:  20023107     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A novel, obligately anaerobic, extremely thermophilic, cellulolytic bacterium, designated OB47(T), was isolated from Obsidian Pool, Yellowstone National Park, WY. The isolate was a nonmotile, non-spore-forming, Gram-positive rod approximately 2 microm long by 0.2 microm wide and grew at temperatures between 55 and 85 degrees C, with the optimum at 78 degrees C. The pH range for growth was 6.0 to 8.0, with values of near 7.0 being optimal. Growth on cellobiose produced the fastest specific growth rate at 0.75 h(-1). The organism also displayed fermentative growth on glucose, maltose, arabinose, fructose, starch, lactose, mannose, sucrose, galactose, xylose, arabinogalactan, Avicel, xylan, filter paper, processed cardboard, pectin, dilute acid-pretreated switchgrass, and Populus. OB47(T) was unable to grow on mannitol, fucose, lignin, Gelrite, acetate, glycerol, ribose, sorbitol, carboxymethylcellulose, and casein. Yeast extract stimulated growth, and thiosulfate, sulfate, nitrate, and sulfur were not reduced. Fermentation end products were mainly acetate, H2, and CO2, although lactate and ethanol were produced in 5-liter batch fermentations. The G+C content of the DNA was 35 mol%, and sequence analysis of the small subunit rRNA gene placed OB47(T) within the genus Caldicellulosiruptor. Based on its phylogenetic and phenotypic properties, the isolate is proposed to be designated Caldicellulosiruptor obsidiansis sp. nov. and OB47 is the type strain (ATCC BAA-2073).
Authors:
Scott D Hamilton-Brehm; Jennifer J Mosher; Tatiana Vishnivetskaya; Mircea Podar; Sue Carroll; Steve Allman; Tommy J Phelps; Martin Keller; James G Elkins
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2009-12-18
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  76     ISSN:  1098-5336     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2010 Feb 
Date Detail:
Created Date:  2010-02-08     Completed Date:  2010-04-02     Revised Date:  2013-05-31    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1014-20     Citation Subset:  IM    
Affiliation:
Biosciences Division, Oak Ridge National Laboratory, P.O. Box 2008 MS6038, Oak Ridge, TN 37831, USA.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/GQ338764
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MeSH Terms
Descriptor/Qualifier:
Bacteria, Anaerobic / classification,  genetics,  isolation & purification*,  metabolism
Base Composition
Base Sequence
Cellobiose / metabolism
DNA, Bacterial / chemistry,  genetics
Fermentation
Genes, Bacterial
Hot Temperature
Microscopy, Electron, Scanning
Molecular Sequence Data
Phenotype
Phylogeny
RNA, Bacterial / genetics
RNA, Ribosomal, 16S / genetics
Water Microbiology*
Wyoming
Chemical
Reg. No./Substance:
0/DNA, Bacterial; 0/RNA, Bacterial; 0/RNA, Ribosomal, 16S; 16462-44-5/Cellobiose
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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