| Calculating optimal modulation periods to maximize the peak capacity in two-dimensional HPLC. | |
| | |
MedLine Citation:
|
PMID: 17437330 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Theoretical calculations are presented to optimize modulation period for maximum total peak capacity in comprehensive two-dimensional HPLC (2D-HPLC) taking into account the effect of modulation on the apparent peak capacity of the first-dimension (1D) separation. Results indicate that modulation periods are most favorable when they are adjusted to approximately 2.2-4 times the standard deviation of a 1D peak in order to avoid excessively short run times at the second dimension (2D). Data are presented that effective peak capacities of several thousand in 60 min can be expected for practical 2D-HPLC conditions, utilizing 1D gradient elution followed by 2D isocratic elution, that remain at approximately 50-70% of the theoretical maximum peak capacity. This work suggests that lower modulation frequencies and longer 2D separation times than previously proposed are favorable under realistic chromatographic conditions, alleviating some practical problems associated with 2D-HPLC. |
| | |
Authors:
|
Kanta Horie; Hiroshi Kimura; Tohru Ikegami; Akira Iwatsuka; Nabil Saad; Oliver Fiehn; Nobuo Tanaka |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-04-17 |
Journal Detail:
|
Title: Analytical chemistry Volume: 79 ISSN: 0003-2700 ISO Abbreviation: Anal. Chem. Publication Date: 2007 May |
Date Detail:
|
Created Date: 2007-05-14 Completed Date: 2007-07-06 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0370536 Medline TA: Anal Chem Country: United States |
Other Details:
|
Languages: eng Pagination: 3764-70 Citation Subset: IM |
Affiliation:
|
Department of Biomolecular Engineering, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Chromatography, High Pressure Liquid
/
methods*,
standards Models, Theoretical* |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Proteomic mapping of 4-hydroxynonenal protein modification sites by solid-phase hydrazide chemistry ...
Next Document: Electrochemistry and electrocatalytic properties of hemoglobin in layer-by-layer films of SiO2 with ...