Document Detail


Calcium-translocating and cardiotonic properties of oxidation products of linoleic acid.
MedLine Citation:
PMID:  4075258     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Calcium-translocating activity of linoleic acid and its lipoxygenase (linoleate: oxygen oxidoreductase; EC 1.13.11.12) metabolites or autoxidation products was determined in vitro by estimation of 45Ca transport from a bulk aqueous to a bulk organic phase. Fresh commercial linoleic acid, tested immediately after removal from a sealed vial, stimulated calcium translocation only at concentrations greater than 1 mM. In contrast, 45Ca translocation by linoleic acid exposed to air was detectable at 10 microM. Oxidation products of linoleic acid obtained either by incubation with lipoxygenase or by autoxidation were much less potent than the calcium ionophore A23187. The products obtained by enzymic oxidation of linoleic acid enhanced contractility in the Langendorff-perfused guinea pig heart up to 45% over control (at 3 X 10(-8) M). The inotropic response was transient with rapid onset and not affected by the beta-adrenergic antagonist, propranolol. The autoxidation products of linoleic acid increased cardiac contractility up to 43% at 10(-6) M. In contrast, fresh linoleic acid caused only a negative inotropic effect at 10(-8) to 3 X 10(-7) M, progressing to contracture at 10(-6) M. These findings suggest that conflicting reports on the cardiostimulant effect of linoleic acid may be due to varying levels of the autoxidation products. Linoleic acid metabolites in vivo may have a physiological role in myocardial function related to their Ca2+-ionophoric activity.
Authors:
R S Kim; I Bihler; F S LaBella
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Canadian journal of physiology and pharmacology     Volume:  63     ISSN:  0008-4212     ISO Abbreviation:  Can. J. Physiol. Pharmacol.     Publication Date:  1985 Nov 
Date Detail:
Created Date:  1986-02-12     Completed Date:  1986-02-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372712     Medline TA:  Can J Physiol Pharmacol     Country:  CANADA    
Other Details:
Languages:  eng     Pagination:  1392-7     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Biological Transport, Active
Calcium / metabolism*
Calcium Radioisotopes / diagnostic use
Cardiotonic Agents*
Guinea Pigs
Hydrogen Peroxide / metabolism
Isoproterenol / pharmacology
Linoleic Acids / metabolism,  pharmacology*
Male
Mitochondria, Heart / metabolism
Myocardial Contraction / drug effects
Oxidation-Reduction
Spectrophotometry, Ultraviolet
Chemical
Reg. No./Substance:
0/Calcium Radioisotopes; 0/Cardiotonic Agents; 0/Linoleic Acids; 7440-70-2/Calcium; 7683-59-2/Isoproterenol; 7722-84-1/Hydrogen Peroxide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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