Document Detail

Calcium and α-tocopherol suppress cured-meat promotion of chemically induced colon carcinogenesis in rats and reduce associated biomarkers in human volunteers.
MedLine Citation:
PMID:  24025632     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Processed meat intake has been associated with increased colorectal cancer risk. We have shown that cured meat promotes carcinogen-induced preneoplastic lesions and increases specific biomarkers in the colon of rats.
OBJECTIVES: We investigated whether cured meat modulates biomarkers of cancer risk in human volunteers and whether specific agents can suppress cured meat-induced preneoplastic lesions in rats and associated biomarkers in rats and humans.
DESIGN: Six additives (calcium carbonate, inulin, rutin, carnosol, α-tocopherol, and trisodium pyrophosphate) were added to cured meat given to groups of rats for 14 d, and fecal biomarkers were measured. On the basis of these results, calcium and tocopherol were kept for the following additional experiments: cured meat, with or without calcium or tocopherol, was given to dimethylhydrazine-initiated rats (47% meat diet for 100 d) and to human volunteers in a crossover study (180 g/d for 4 d). Rat colons were scored for mucin-depleted foci, putative precancer lesions. Biomarkers of nitrosation, lipoperoxidation, and cytotoxicity were measured in the urine and feces of rats and volunteers.
RESULTS: Cured meat increased nitroso compounds and lipoperoxidation in human stools (both P < 0.05). Calcium normalized both biomarkers in rats and human feces, whereas tocopherol only decreased nitro compounds in rats and lipoperoxidation in feces of volunteers (all P < 0.05). Last, calcium and tocopherol reduced the number of mucin-depleted foci per colon in rats compared with nonsupplemented cured meat (P = 0.01).
CONCLUSION: Data suggest that the addition of calcium carbonate to the diet or α-tocopherol to cured meat may reduce colorectal cancer risk associated with cured-meat intake. This trial was registered at as NCT00994526.
Fabrice H F Pierre; Océane C B Martin; Raphaelle L Santarelli; Sylviane Taché; Nathalie Naud; Françoise Guéraud; Marc Audebert; Jacques Dupuy; Nathalie Meunier; Didier Attaix; Jean-Luc Vendeuvre; Sidney S Mirvish; Gunter C G Kuhnle; Noel Cano; Denis E Corpet
Publication Detail:
Type:  Journal Article; Randomized Controlled Trial; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2013-09-11
Journal Detail:
Title:  The American journal of clinical nutrition     Volume:  98     ISSN:  1938-3207     ISO Abbreviation:  Am. J. Clin. Nutr.     Publication Date:  2013 Nov 
Date Detail:
Created Date:  2013-10-21     Completed Date:  2013-12-12     Revised Date:  2014-11-04    
Medline Journal Info:
Nlm Unique ID:  0376027     Medline TA:  Am J Clin Nutr     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1255-62     Citation Subset:  AIM; IM    
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MeSH Terms
Acetylcysteine / urine
Biological Markers / blood
Blood Glucose / analysis
C-Reactive Protein / analysis,  metabolism
Calcium, Dietary / administration & dosage*
Carcinogenesis / chemically induced,  pathology*
Carcinogens / toxicity
Cholesterol / blood
Colon / drug effects*,  pathology
Colorectal Neoplasms / drug therapy,  prevention & control
Creatinine / blood
Cross-Over Studies
Dimethylhydrazines / administration & dosage,  adverse effects
Diphosphates / administration & dosage
Diterpenes, Abietane / administration & dosage
Feces / chemistry
Healthy Volunteers
Inulin / administration & dosage
Meat Products / adverse effects*
Middle Aged
Rats, Inbred F344
Rutin / administration & dosage
Single-Blind Method
Thiobarbituric Acid Reactive Substances / analysis,  metabolism
alpha-Tocopherol / administration & dosage*
Grant Support
R01-CA-1434600/CA/NCI NIH HHS
Reg. No./Substance:
0/Biological Markers; 0/Blood Glucose; 0/Calcium, Dietary; 0/Carcinogens; 0/Dimethylhydrazines; 0/Diphosphates; 0/Diterpenes, Abietane; 0/Thiobarbituric Acid Reactive Substances; 5957-80-2/carnosol; 5G06TVY3R7/Rutin; 9005-80-5/Inulin; 9007-41-4/C-Reactive Protein; 97C5T2UQ7J/Cholesterol; AYI8EX34EU/Creatinine; H4N855PNZ1/alpha-Tocopherol; O352864B8Z/sodium pyrophosphate; WYQ7N0BPYC/Acetylcysteine

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