Document Detail


Calcium tips the balance: a microtubule plus end to lattice binding switch operates in the carboxyl terminus of BPAG1n4.
MedLine Citation:
PMID:  22995871     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Microtubules (MTs) are integral to numerous cellular functions, such as cell adhesion, differentiation and intracellular transport. Their dynamics are largely controlled by diverse MT-interacting proteins, but the signalling mechanisms that regulate these interactions remain elusive. In this report, we identify a rapid, calcium-regulated switch between MT plus end interaction and lattice binding within the carboxyl terminus of BPAG1n4. This switch is EF-hand dependent, and mutations of the EF-hands abolish this dynamic behaviour. Our study thus uncovers a new, calcium-dependent regulatory mechanism for a spectraplakin, BPAG1n4, at the MT plus end.
Authors:
Mridu Kapur; Wei Wang; Michael T Maloney; Ivan Millan; Victor F Lundin; Thuy-An Tran; Yanmin Yang
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-09-21
Journal Detail:
Title:  EMBO reports     Volume:  13     ISSN:  1469-3178     ISO Abbreviation:  EMBO Rep.     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-06     Completed Date:  2013-07-29     Revised Date:  2013-11-05    
Medline Journal Info:
Nlm Unique ID:  100963049     Medline TA:  EMBO Rep     Country:  England    
Other Details:
Languages:  eng     Pagination:  1021-9     Citation Subset:  IM    
Affiliation:
Department of Neurology and Neurological Sciences, Stanford University School of Medicine, MSLS Building, Room P259, 1201 Welch Road, Stanford, California 94305, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
COS Cells
Calcium / metabolism*
Carrier Proteins / chemistry,  genetics,  metabolism*
Cercopithecus aethiops
Cytoskeletal Proteins / chemistry,  genetics,  metabolism*
EF Hand Motifs
HEK293 Cells
Humans
Microtubules / chemistry,  metabolism*
Mutation
Nerve Tissue Proteins / chemistry,  genetics,  metabolism*
Chemical
Reg. No./Substance:
0/Carrier Proteins; 0/Cytoskeletal Proteins; 0/DST protein, human; 0/Nerve Tissue Proteins; 7440-70-2/Calcium
Comments/Corrections

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