Document Detail

Calcium stimulation of plasminogen activator secretion/production by swiss 3T3 cells.
MedLine Citation:
PMID:  197085     Owner:  NLM     Status:  MEDLINE    
Actively growing Swiss 3T3 cells secret high levels of plasminogen activator which decreases after the cells become confluent. In contrast, simian virus 40-transformed 3T3 cells secrete large amounts of plasminogen activator independent of cell density (Chou, I.-N., O'Donnel, S.P., Black, P.H., and Roblin, R.O. (1977) J. Cell. Physiol. 91, 31-38). These results suggest a correlation between active cell multiplication and plasminogen activator secretion in both 3T3 and simian virus-transformed 3T3 cells. The data reported herein indicate that treatment of both subconfluent and confluent Swiss 3T3 cells with high concentrations of Ca2+ (final 3.0 to 4.9 mM) increases the amounts of both secreted and cell-associated plasminogen activator in a dose-dependent manner. In addition, the ionophore A23187 (19 to 95 nM) in the presence of a normal level of Ca2+ (1.8 mM) stimulates both production and secretion of plasminogen activator from growing 3T3 cells. The Ca2+ stimulation of plasminogen activator production/secretion may be related to the mitogenic effect of Ca2+.
I N Chou; R O Roblin; P H Black
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  252     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  1977 Sep 
Date Detail:
Created Date:  1977-10-28     Completed Date:  1977-10-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  6256-9     Citation Subset:  IM    
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MeSH Terms
Calcimycin / pharmacology
Calcium / pharmacology*
Cell Transformation, Neoplastic
Cells, Cultured
Plasminogen Activators / metabolism*
Simian virus 40
Stimulation, Chemical
Reg. No./Substance:
52665-69-7/Calcimycin; 7440-70-2/Calcium; EC 3.4.21.-/Plasminogen Activators

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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