Document Detail

Calcium sensitivity of myofilaments in cardiac muscle--effect of myosin phosphorylation.
MedLine Citation:
PMID:  3024616     Owner:  NLM     Status:  MEDLINE    
We investigated the influence of the extent of phosphorylation of the myosin P-light-chain on the calcium sensitivity of skinned heart fibres. Treatment of skinned heart fibres with PCM-phosphatase decreased the phosphorylation level of P-light-chain from 0.3 mol P/mol LC-2 and 0.2 mol P/mol LC-2* to 0.16 mol P/mol LC-2 and 0.14 mol P/mol LC-2*. Isometric tension development decreased by 34% at submaximal Ca2+-concentration (pCa 5.5) after incubation with PCM-phosphatase while tension achieved at maximum Ca2+-concentration (pCa 4.3) was not affected. The effect of desensitization on skinned fibres could be reversed by washing out the phosphatase or by addition of myosin light chain kinase.
I Morano; J C Rüegg
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Basic research in cardiology     Volume:  81 Suppl 1     ISSN:  0300-8428     ISO Abbreviation:  Basic Res. Cardiol.     Publication Date:  1986  
Date Detail:
Created Date:  1987-01-21     Completed Date:  1987-01-21     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0360342     Medline TA:  Basic Res Cardiol     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  17-23     Citation Subset:  IM    
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MeSH Terms
Calcium / pharmacology*
Cytoskeleton / metabolism*
Heart / drug effects*
Microfilaments / metabolism*
Myocardium / enzymology,  metabolism*
Myosin-Light-Chain Kinase / pharmacology
Myosin-Light-Chain Phosphatase
Myosins / metabolism*
Phosphoprotein Phosphatases / metabolism*
Reg. No./Substance:
7440-70-2/Calcium; EC Kinase; EC Phosphatases; EC Phosphatase; EC

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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