Document Detail


Calcium oxalate crystals in fetal bovine serum: implications for cell culture, phagocytosis and biomineralization studies in vitro.
MedLine Citation:
PMID:  17879965     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cell culture methods and models are key investigative tools for cell and molecular biology studies. Fetal bovine serum (FBS) is commonly used as an additive during cell culture since its constituents promote cell survival, proliferation and differentiation. Here we report that commercially available FBS from different major suppliers consistently contain precipitated, calcium oxalate crystals-either in the monohydrate (COM) or dihydrate (COD) form. Mineral structure and phase identification of the crystals were determined by X-ray diffraction, chemical composition by energy-dispersive X-ray microanalysis, and imaging and measurement of crystal growth steps by atomic force microscopy-all identified and confirmed crystallographic parameters for COM and COD. Proteins binding to the crystals were identified by immunoblotting, revealing the presence of osteopontin and fetuin-A (alpha(2)HS-glycoprotein)--known regulators of crystal growth found in serum. Macrophage cell cultures exposed to calcium oxalate crystals showed internalization of the crystals by phagocytosis in a process that induced disruption of cell-cell adhesion, release of reactive oxygen species and membrane damage, events that may be linked to the release of inflammatory cytokines by these cells into the culture media. In conclusion, calcium oxalate crystals found in commercially available FBS are toxic to cells, and their presence may confound results from in vitro studies where, amongst others, phagocytosis, biomineralization, renal cell and molecular biology, and drug and biomaterial testing are being examined.
Authors:
Claudio E Pedraza; Yung-Ching Chien; Marc D McKee
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of cellular biochemistry     Volume:  103     ISSN:  1097-4644     ISO Abbreviation:  J. Cell. Biochem.     Publication Date:  2008 Apr 
Date Detail:
Created Date:  2008-04-02     Completed Date:  2008-07-16     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8205768     Medline TA:  J Cell Biochem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1379-93     Citation Subset:  IM    
Copyright Information:
2007 Wiley-Liss, Inc.
Affiliation:
Faculty of Dentistry, McGill University, Montreal, Quebec, Canada H3A 2B2.
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MeSH Terms
Descriptor/Qualifier:
Animals
Blood Proteins / chemistry
Calcium Oxalate / chemistry*
Cattle
Cell Culture Techniques*
Cell Line
Macrophages / cytology*
Mice
NIH 3T3 Cells
Osteopontin / chemistry
Phagocytosis*
Serum / chemistry*
Chemical
Reg. No./Substance:
0/Blood Proteins; 0/alpha2HS glycoprotein; 106441-73-0/Osteopontin; 25454-23-3/Calcium Oxalate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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