Document Detail


Calcium- and myristoyl-dependent subcellular localization of the neuronal calcium-binding protein VILIP in transfected PC12 cells.
MedLine Citation:
PMID:  9147390     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Wild-type neuronal calcium-binding protein VILIP (visinin-like protein), and a myristoylation mutant of VILIP which lacks the consensus sequence for N-terminal myristoylation, have been stably transfected in PC12 cells. Immunocytochemical studies of VILIP-transfected PC12 cells have revealed the wild-type VILIP is strongly concentrated at the cell membrane, particularly at cell-cell contact sites, but is also distributed throughout the cytosol at moderate levels. In contrast, myristoylation-mutant VILIP shows a more even distribution, with significantly less association at cell-cell contact sites. Western blot analysis of subcellular fractions has shown that wild-type VILIP associates in a calcium-dependent manner with membrane fractions, whereas the myristoylation mutant only weakly associates with this fraction. Therefore, a calcium-myristoyl switch seems to be a major, but not sole determinant for the association of VILIP with membranes in living cells.
Authors:
C Spilker; E D Gundelfinger; K H Braunewell
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Neuroscience letters     Volume:  225     ISSN:  0304-3940     ISO Abbreviation:  Neurosci. Lett.     Publication Date:  1997 Apr 
Date Detail:
Created Date:  1997-07-24     Completed Date:  1997-07-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7600130     Medline TA:  Neurosci Lett     Country:  IRELAND    
Other Details:
Languages:  eng     Pagination:  126-8     Citation Subset:  IM    
Affiliation:
Department of Molecular Biology and Neurochemistry, Federal Institute for Neurobiology, Magdeburg, Germany.
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MeSH Terms
Descriptor/Qualifier:
Animals
Calcium / pharmacology*
Calcium-Binding Proteins / metabolism*
Immunohistochemistry
Nerve Tissue Proteins / metabolism*
Neurocalcin
Neurons / drug effects*,  metabolism
PC12 Cells / metabolism*
Rats
Receptors, Calcium-Sensing*
Transfection
Chemical
Reg. No./Substance:
0/Calcium-Binding Proteins; 0/Nerve Tissue Proteins; 0/Neurocalcin; 0/Receptors, Calcium-Sensing; 0/Vsnl1 protein, rat; 7440-70-2/Calcium

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