Document Detail

Calcium mobilization during nicotine-induced cell death in human glioma and glioblastoma cell lines.
MedLine Citation:
PMID:  9703899     Owner:  NLM     Status:  MEDLINE    
Nicotine dose-dependently induced cytotoxicity in human glioma (KG-1-C) and glioblastoma (GBS-1, T98G) cell lines, but could not induce internucleosomal DNA cleavage, in contrast to apoptosing human myelogenous leukemic cell lines. Human glioma/glioblastoma cell lines thus might have a chromatin structure resistant to endonuclease digestion. Nicotine induced a rapid increase in the intracellular calcium concentration. Confocal experiments with Fluo-3 fluorescence revealed that nicotine elevated the free Ca2+ concentration in both nuclear and cytoplasmic regions of the cells, and the elevation of Ca2+ in the nuclear region was more pronounced than that of the cytoplasmic region. The present study suggests that nuclear accumulation of Ca2+ is an important initial step for cell death induction by nicotine.
M Yamamura; Y Amano; H Sakagami; Y Yamanaka; Y Nishimoto; H Yoshida; M Yamaguchi; H Ohata; K Momose; M Takeda
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Anticancer research     Volume:  18     ISSN:  0250-7005     ISO Abbreviation:  Anticancer Res.     Publication Date:    1998 Jul-Aug
Date Detail:
Created Date:  1998-09-01     Completed Date:  1998-09-01     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8102988     Medline TA:  Anticancer Res     Country:  GREECE    
Other Details:
Languages:  eng     Pagination:  2499-502     Citation Subset:  IM    
Department of Biochemistry, School of Medicine, School of Pharmaceutical Sciences, Showa University, Tokyo, Japan.
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MeSH Terms
Apoptosis / drug effects*,  physiology
Brain Neoplasms
Calcium / metabolism*
Cell Survival / drug effects*
DNA Fragmentation
Dose-Response Relationship, Drug
Nicotine / toxicity*
Time Factors
Tumor Cells, Cultured
Reg. No./Substance:
54-11-5/Nicotine; 7440-70-2/Calcium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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