| Calcium ionophoretic activity of chemically synthesized oligomeric derivatives of prostaglandin B1. | |
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MedLine Citation:
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PMID: 3828398 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Chemically synthesized dimers, trimers and tetramers of 15-dehydroprostaglandin B1 and 16,16'-dimethyl-15-dehydroprostaglandin B1 facilitate the release of Ca2+ from isolated rat liver mitochondria. The parent monomeric prostaglandins had no significant activity. The rate of release was stimulated by exogenous K+ or Na+, suggesting an antiport exchange of monovalent cations for intra-mitochondrial Ca2+. The activity depended upon the presence of ruthenium red, which prevented recycling of Ca2+; comparison of the activity with A23187 and carbonyl cyanide p-trifluoromethoxyphenylhydrazone indicated that the prostaglandin B1 oligomers were functioning as ionophores and the release of Ca2+ was not caused by an uncoupling of oxidative phosphorylation. The oligomers caused a major decrease in the membrane potential but only when the mitochondria were preloaded with exogenous Ca2+, and even then, the Ca2+ efflux was completed before the membrane potential decreased to less than 90 mV. The oligomeric molecules were able to form supramolecular aggregates in the presence of Ca2+ as detected by light scattering. They extracted Ca2+ into an organic phase, and translocated Ca2+ from one aqueous domain to another across an organic barrier; K+ and Na+ modulated these processes. The prostaglandin B1 derivatives also translocated Rb+ from one aqueous phase to another across an organic barrier when Ca2+ was translocated. |
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Authors:
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S Uribe; S T Ohnishi; C Israelite; T M Devlin |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Biochimica et biophysica acta Volume: 924 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 1987 Apr |
Date Detail:
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Created Date: 1987-05-21 Completed Date: 1987-05-21 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 87-98 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Calcium / metabolism* Intracellular Membranes / drug effects, physiology Ionophores / pharmacology* Kinetics Male Membrane Potentials / drug effects Mitochondria, Liver / drug effects, metabolism* Potassium / pharmacology Prostaglandins / pharmacology* Prostaglandins B / chemical synthesis, pharmacology* Rats Rats, Inbred Strains Sodium / pharmacology Structure-Activity Relationship |
| Chemical | |
Reg. No./Substance:
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0/16,16-dimethyl-15-dehydroprostaglandin B1 trimer; 0/Ionophores; 0/Prostaglandins; 0/Prostaglandins B; 7440-09-7/Potassium; 7440-23-5/Sodium; 7440-70-2/Calcium |
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