Document Detail


Calcium ionophore, A23187 and its amino acid complexes: spectroscopic and molecular modeling studies.
MedLine Citation:
PMID:  7946064     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The circular dichroism, fluorescence, Nuclear Magnetic Resonance and BLM conductance studies indicate that A23187 forms a stable complex with amino acids at low ionophore concentrations (< 10(-4)M). However, A23187 prefers to be in a dimeric structure with no significant binding to amino acids, at concentrations higher than 10(-4)M. It was also observed that at lower concentrations, at which the amino acids bind to the ionophore, the affinity for calcium ions was several orders of magnitude lower than that at higher ionophore concentrations. We have also conducted molecular modeling studies to examine the structure of the A23187 dimer and its amino acid complexes. The results of these modeling studies strongly support our experimental results and validate the formation of a hydrogen bonded and energetically stable A23187 dimer and its amino acid complexes.
Authors:
K Namboodiri; B P Gaber; K R Easwaran; S V Balasubramanian
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Journal of biomolecular structure & dynamics     Volume:  11     ISSN:  0739-1102     ISO Abbreviation:  J. Biomol. Struct. Dyn.     Publication Date:  1994 Apr 
Date Detail:
Created Date:  1994-12-06     Completed Date:  1994-12-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8404176     Medline TA:  J Biomol Struct Dyn     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  913-26     Citation Subset:  IM    
Affiliation:
Center for Bio-Molecular Science and Engineering, Naval Research Laboratory, Washington, D.C. 20375.
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MeSH Terms
Descriptor/Qualifier:
Amino Acids / metabolism*
Binding Sites
Calcimycin / analysis,  chemistry*,  metabolism
Circular Dichroism
Hydrogen Bonding
Magnetic Resonance Spectroscopy
Models, Molecular
Phosphatidylcholines / metabolism
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Phosphatidylcholines; 52665-69-7/Calcimycin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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