| Calcium inflow of hamster Merkel cells in response to hyposmotic stimulation indicate a stretch activated ion channel. | |
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MedLine Citation:
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PMID: 9535115 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The aim of this study was to investigate the existence of the stretch activated ion channels in single Merkel cell using microfluorimetric techniques. Single Merkel cells were dissociated enzymatically from the touch domes in the cheek pouch mucosa of 4-8 week old golden hamsters. They were loaded with calcium (Ca2+) fluorescent indicator fura-2/AM. The increase in intracellular Ca2+ concentration ([Ca2+]i) of a single Merkel cell (quinacrine fluorescent cell) was induced by hyposmotic solution in normal Krebs solution, while it was not induced by Ca2+-free hyposmotic solution in Ca2+-free physiological solution. Gadolinium ion (10 microM) in normal Krebs solution partially blocked the increase in [Ca2+]i of Merkel cells induced by hyposmotic solution. Hence, this study revealed that stretch activated ion channels existed on the Merkel cell membrane. |
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Authors:
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M Tazaki; T Suzuki |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Neuroscience letters Volume: 243 ISSN: 0304-3940 ISO Abbreviation: Neurosci. Lett. Publication Date: 1998 Feb |
Date Detail:
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Created Date: 1998-05-14 Completed Date: 1998-05-14 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7600130 Medline TA: Neurosci Lett Country: IRELAND |
Other Details:
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Languages: eng Pagination: 69-72 Citation Subset: IM |
Affiliation:
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Department of Physiology, Oral Health Science Center, Tokyo Dental College, Chiba, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Calcium / metabolism*, pharmacokinetics Calcium Channels / physiology* Cricetinae Gadolinium / pharmacology Hypertonic Solutions Ion Channel Gating / physiology Isotonic Solutions / pharmacology Merkel Cells / chemistry*, physiology* Mesocricetus Mucous Membrane / chemistry Osmotic Pressure Skin / innervation* Touch / physiology |
| Chemical | |
Reg. No./Substance:
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0/Calcium Channels; 0/Hypertonic Solutions; 0/Isotonic Solutions; 0/Krebs-Ringer solution; 7440-54-2/Gadolinium; 7440-70-2/Calcium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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