Document Detail

Calcium inflow of hamster Merkel cells in response to hyposmotic stimulation indicate a stretch activated ion channel.
MedLine Citation:
PMID:  9535115     Owner:  NLM     Status:  MEDLINE    
The aim of this study was to investigate the existence of the stretch activated ion channels in single Merkel cell using microfluorimetric techniques. Single Merkel cells were dissociated enzymatically from the touch domes in the cheek pouch mucosa of 4-8 week old golden hamsters. They were loaded with calcium (Ca2+) fluorescent indicator fura-2/AM. The increase in intracellular Ca2+ concentration ([Ca2+]i) of a single Merkel cell (quinacrine fluorescent cell) was induced by hyposmotic solution in normal Krebs solution, while it was not induced by Ca2+-free hyposmotic solution in Ca2+-free physiological solution. Gadolinium ion (10 microM) in normal Krebs solution partially blocked the increase in [Ca2+]i of Merkel cells induced by hyposmotic solution. Hence, this study revealed that stretch activated ion channels existed on the Merkel cell membrane.
M Tazaki; T Suzuki
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Neuroscience letters     Volume:  243     ISSN:  0304-3940     ISO Abbreviation:  Neurosci. Lett.     Publication Date:  1998 Feb 
Date Detail:
Created Date:  1998-05-14     Completed Date:  1998-05-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7600130     Medline TA:  Neurosci Lett     Country:  IRELAND    
Other Details:
Languages:  eng     Pagination:  69-72     Citation Subset:  IM    
Department of Physiology, Oral Health Science Center, Tokyo Dental College, Chiba, Japan.
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MeSH Terms
Calcium / metabolism*,  pharmacokinetics
Calcium Channels / physiology*
Gadolinium / pharmacology
Hypertonic Solutions
Ion Channel Gating / physiology
Isotonic Solutions / pharmacology
Merkel Cells / chemistry*,  physiology*
Mucous Membrane / chemistry
Osmotic Pressure
Skin / innervation*
Touch / physiology
Reg. No./Substance:
0/Calcium Channels; 0/Hypertonic Solutions; 0/Isotonic Solutions; 0/Krebs-Ringer solution; 7440-54-2/Gadolinium; 7440-70-2/Calcium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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