Document Detail


Calcium-independent phospholipase A2 regulates retinal pigment epithelium proliferation and may be important in the pathogenesis of retinal diseases.
MedLine Citation:
PMID:  19379734     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Calcium-independent phospholipase A2, group VIA (iPLA2-VIA) is involved in cell proliferation. This study aimed to evaluate the role of iPLA2-VIA in retinal pigment epithelium (RPE) cell proliferation and in retinal diseases involving RPE proliferation. A human RPE cell line (ARPE-19) was used to explore this role in vitro. Proliferating ARPE-19 cells had increased expression and activity of iPLA2-VIA. iPLA2-VIA was found in the nuclei of proliferating ARPE-19 cells, whereas in confluent ARPE-19 cells, with limited proliferation, iPLA2-VIA was primarily found in the cytosol. Inhibition of iPLA2-VIA decreased the rate of proliferation, whereas over expression of iPLA2-VIA increased the rate of proliferation. Using an experimental porcine model of RPE proliferation we demonstrated significant nuclear upregulation of iPLA2-VIA in proliferating RPE cells in vivo. We furthermore evaluated the expression of iPLA2-VIA in proliferative vitreoretinopathy (PVR). PVR membranes revealed nuclear expression of iPLA2-VIA in the RPE cells which had migrated and participated in the formation of the membranes. Overall, the present results point to an important role of iPLA2-VIA in the regulation of RPE proliferation suggesting that iPLA2-VIA may be considered as a possible pharmaceutical target in retinal diseases involving RPE proliferation and migration.
Authors:
M Kolko; J F Kiilgaard; J Wang; K A Poulsen; J R Andreasen; M la Cour; M H Nissen; S Heegaard; N G Bazan; J U Prause
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2009-04-18
Journal Detail:
Title:  Experimental eye research     Volume:  89     ISSN:  1096-0007     ISO Abbreviation:  Exp. Eye Res.     Publication Date:  2009 Sep 
Date Detail:
Created Date:  2009-08-03     Completed Date:  2009-10-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370707     Medline TA:  Exp Eye Res     Country:  England    
Other Details:
Languages:  eng     Pagination:  383-91     Citation Subset:  IM    
Affiliation:
Department of Neuroscience and Pharmacology, Eye Pathology Section, University of Copenhagen, Frederik d. V's vej 11, 1st floor, 2100 Copenhagen, Denmark. mkolko@dadlnet.dk
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MeSH Terms
Descriptor/Qualifier:
Alternative Splicing
Animals
Cell Nucleus / enzymology,  metabolism
Cell Proliferation
Cells, Cultured
Endoplasmic Reticulum / enzymology
Gene Silencing
Humans
Phospholipases A2, Calcium-Independent / genetics,  physiology*
RNA, Small Interfering / genetics
Retinal Pigment Epithelium / cytology*,  enzymology,  pathology
Sus scrofa
Vitreoretinopathy, Proliferative / enzymology*,  pathology
Grant Support
ID/Acronym/Agency:
EY0-05121/EY/NEI NIH HHS
Chemical
Reg. No./Substance:
0/RNA, Small Interfering; EC 3.1.1.4/Phospholipases A2, Calcium-Independent

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