| Calcium-independent phospholipase A2 regulates retinal pigment epithelium proliferation and may be important in the pathogenesis of retinal diseases. | |
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MedLine Citation:
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PMID: 19379734 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Calcium-independent phospholipase A2, group VIA (iPLA2-VIA) is involved in cell proliferation. This study aimed to evaluate the role of iPLA2-VIA in retinal pigment epithelium (RPE) cell proliferation and in retinal diseases involving RPE proliferation. A human RPE cell line (ARPE-19) was used to explore this role in vitro. Proliferating ARPE-19 cells had increased expression and activity of iPLA2-VIA. iPLA2-VIA was found in the nuclei of proliferating ARPE-19 cells, whereas in confluent ARPE-19 cells, with limited proliferation, iPLA2-VIA was primarily found in the cytosol. Inhibition of iPLA2-VIA decreased the rate of proliferation, whereas over expression of iPLA2-VIA increased the rate of proliferation. Using an experimental porcine model of RPE proliferation we demonstrated significant nuclear upregulation of iPLA2-VIA in proliferating RPE cells in vivo. We furthermore evaluated the expression of iPLA2-VIA in proliferative vitreoretinopathy (PVR). PVR membranes revealed nuclear expression of iPLA2-VIA in the RPE cells which had migrated and participated in the formation of the membranes. Overall, the present results point to an important role of iPLA2-VIA in the regulation of RPE proliferation suggesting that iPLA2-VIA may be considered as a possible pharmaceutical target in retinal diseases involving RPE proliferation and migration. |
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Authors:
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M Kolko; J F Kiilgaard; J Wang; K A Poulsen; J R Andreasen; M la Cour; M H Nissen; S Heegaard; N G Bazan; J U Prause |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2009-04-18 |
Journal Detail:
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Title: Experimental eye research Volume: 89 ISSN: 1096-0007 ISO Abbreviation: Exp. Eye Res. Publication Date: 2009 Sep |
Date Detail:
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Created Date: 2009-08-03 Completed Date: 2009-10-08 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0370707 Medline TA: Exp Eye Res Country: England |
Other Details:
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Languages: eng Pagination: 383-91 Citation Subset: IM |
Affiliation:
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Department of Neuroscience and Pharmacology, Eye Pathology Section, University of Copenhagen, Frederik d. V's vej 11, 1st floor, 2100 Copenhagen, Denmark. mkolko@dadlnet.dk |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Alternative Splicing Animals Cell Nucleus / enzymology, metabolism Cell Proliferation Cells, Cultured Endoplasmic Reticulum / enzymology Gene Silencing Humans Phospholipases A2, Calcium-Independent / genetics, physiology* RNA, Small Interfering / genetics Retinal Pigment Epithelium / cytology*, enzymology, pathology Sus scrofa Vitreoretinopathy, Proliferative / enzymology*, pathology |
| Grant Support | |
ID/Acronym/Agency:
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EY0-05121/EY/NEI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/RNA, Small Interfering; EC 3.1.1.4/Phospholipases A2, Calcium-Independent |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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