| Calcium is essential for the major pseudopilin in the type 2 secretion system. | |
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MedLine Citation:
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PMID: 19640838 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The pseudopilus is a key feature of the type 2 secretion system (T2SS) and is made up of multiple pseudopilins that are similar in fold to the type 4 pilins. However, pilins have disulfide bridges, whereas the major pseudopilins of T2SS do not. A key question is therefore how the pseudopilins, and in particular, the most abundant major pseudopilin, GspG, obtain sufficient stability to perform their function. Crystal structures of Vibrio cholerae, Vibrio vulnificus, and enterohemorrhagic Escherichia coli (EHEC) GspG were elucidated, and all show a calcium ion bound at the same site. Conservation of the calcium ligands fully supports the suggestion that calcium ion binding by the major pseudopilin is essential for the T2SS. Functional studies of GspG with mutated calcium ion-coordinating ligands were performed to investigate this hypothesis and show that in vivo protease secretion by the T2SS is severely impaired. Taking all evidence together, this allows the conclusion that, in complete contrast to the situation in the type 4 pili system homologs, in the T2SS, the major protein component of the central pseudopilus is dependent on calcium ions for activity. |
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Authors:
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Konstantin V Korotkov; Miranda D Gray; Allison Kreger; Stewart Turley; Maria Sandkvist; Wim G J Hol |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2009-07-29 |
Journal Detail:
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Title: The Journal of biological chemistry Volume: 284 ISSN: 1083-351X ISO Abbreviation: J. Biol. Chem. Publication Date: 2009 Sep |
Date Detail:
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Created Date: 2009-09-14 Completed Date: 2009-10-19 Revised Date: 2010-09-21 |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: United States |
Other Details:
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Languages: eng Pagination: 25466-70 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, Biomolecular Structure Center, University of Washington, Seattle, Washington 98195, USA. |
| Data Bank Information | |
Bank Name/Acc. No.:
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PDB/3FU1; 3G20; 3GN9 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Biological Transport
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physiology Calcium / chemistry*, metabolism Cations, Divalent / chemistry, metabolism Enterohemorrhagic Escherichia coli / chemistry*, genetics, metabolism Fimbriae Proteins / chemistry*, genetics, metabolism Ligands Mutation Protein Binding / physiology Protein Stability Protein Structure, Tertiary / physiology Structural Homology, Protein Structure-Activity Relationship Vibrio cholerae / chemistry*, genetics, metabolism |
| Grant Support | |
ID/Acronym/Agency:
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AI34501/AI/NIAID NIH HHS; AI49294/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Cations, Divalent; 0/Ligands; 147680-16-8/Fimbriae Proteins; 7440-70-2/Calcium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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