Document Detail


Calcium-dependent regulation of cyclic photosynthetic electron transfer by a CAS, ANR1, and PGRL1 complex.
MedLine Citation:
PMID:  23045639     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cyclic photosynthetic electron flow (CEF) is crucial to photosynthesis because it participates in the control of chloroplast energy and redox metabolism, and it is particularly induced under adverse environmental conditions. Here we report that down-regulation of the chloroplast localized Ca(2+) sensor (CAS) protein by an RNAi approach in Chlamydomonas reinhardtii results in strong inhibition of CEF under anoxia. Importantly, this inhibition is rescued by an increase in the extracellular Ca(2+) concentration, inferring that CEF is Ca(2+)-dependent. Furthermore, we identified a protein, anaerobic response 1 (ANR1), that is also required for effective acclimation to anaerobiosis. Depletion of ANR1 by artificial microRNA expression mimics the CAS-depletion phenotype, and under anaerobic conditions the two proteins coexist within a large active photosystem I-cytochrome b(6)/f complex. Moreover, we provide evidence that CAS and ANR1 interact with each other as well as with PGR5-Like 1 (PGRL1) in vivo. Overall our data establish a Ca(2+)-dependent regulation of CEF via the combined function of ANR1, CAS, and PGRL1, associated with each other in a multiprotein complex.
Authors:
Mia Terashima; Dimitris Petroutsos; Meike Hüdig; Irina Tolstygina; Kerstin Trompelt; Philipp Gäbelein; Christian Fufezan; Jörg Kudla; Stefan Weinl; Giovanni Finazzi; Michael Hippler
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-10-08
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  109     ISSN:  1091-6490     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-24     Completed Date:  2013-01-07     Revised Date:  2013-07-11    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  17717-22     Citation Subset:  IM    
Affiliation:
Institute of Plant Biology and Biotechnology, University of Münster, 48143 Münster, Germany.
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MeSH Terms
Descriptor/Qualifier:
Animals
Caenorhabditis elegans / metabolism,  physiology
Caenorhabditis elegans Proteins / metabolism,  physiology*
Electron Transport
Electrophoresis, Polyacrylamide Gel
Photosynthesis*
Spectrometry, Fluorescence
Chemical
Reg. No./Substance:
0/Caenorhabditis elegans Proteins
Comments/Corrections
Erratum In:
Proc Natl Acad Sci U S A. 2013 Feb 19;110(8):3198

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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