| Calcium-dependent incorporation of choline into phosphatidylcholine (PC) by base-exchange in rat brain membranes occurs preferentially with phospholipid substrates containing docosahexaenoic acid (22:6(n-3)). | |
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MedLine Citation:
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PMID: 2171666 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The fatty acid composition of phosphatidylcholine (PC) formed by base-exchange was examined in rat brain membranes in vitro. The free choline incorporated into subspecies of PC by this phospholipase-D type activity can be distinguished from that which might enter indirectly via the last enzyme of the de novo pathway for phospholipid biosynthesis, cholinephosphotransferase, by its ionic requirements. Choline base-exchange in lysed synaptosomes is optimal when assayed at extracellular (mM) calcium concentrations and is blocked by magnesium. As much as 40% of the choline incorporated by base-exchange into rat brain membranes was recovered in subspecies of PC, representing no more than 10% of the total PC pool, which contained docosahexaenoic acid (22:6(n-3)). Docosahexaenoic acid is enriched in electrically-excitable membranes and its content in phospholipids of rat and human brain change during early development and increase with age. |
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Authors:
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P G Holbrook; R J Wurtman |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Biochimica et biophysica acta Volume: 1046 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 1990 Sep |
Date Detail:
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Created Date: 1990-12-04 Completed Date: 1990-12-04 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 185-8 Citation Subset: IM |
Affiliation:
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Department of Brain and Cognitive Sciences E25-604, Massachusetts Institute of Technology, Cambridge. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Brain / drug effects, metabolism* Brain Chemistry Calcium / pharmacology* Cell Membrane / metabolism Choline / metabolism* Diacylglycerol Cholinephosphotransferase / metabolism Docosahexaenoic Acids / analysis*, metabolism Magnesium / pharmacology Male Phosphatidylcholines / metabolism* Phospholipase D / metabolism Phospholipids / analysis Rats Rats, Inbred Strains Synaptosomes / drug effects, metabolism |
| Grant Support | |
ID/Acronym/Agency:
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1 F31 MH09199-01/MH/NIMH NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Phosphatidylcholines; 0/Phospholipids; 25167-62-8/Docosahexaenoic Acids; 62-49-7/Choline; 7439-95-4/Magnesium; 7440-70-2/Calcium; EC 2.7.8.2/Diacylglycerol Cholinephosphotransferase; EC 3.1.4.4/Phospholipase D |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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