Document Detail

Calcium dependence of human sperm fertilizing ability.
MedLine Citation:
PMID:  3392517     Owner:  NLM     Status:  MEDLINE    
The Ca2+ dependency of human-sperm fertilizing ability was investigated using a modified Tyrode's medium either containing 2.4 mM CaCl2 (CA medium) or with the CaCl2 replaced by SrCl2 and 0.1 mM EGTA added to chelate any residual Ca2+ ions (SREG-medium). Ten washed sperm populations incubated in either medium for 0, 6, and 22 hours showed the same occurrence of acrosome reactions (by fluorescent lectin labelling and triple stain). A further 3-hour incubation after washing into fresh CA medium resulted in only a slight increase in acrosome reactions in both media. Eight sperm populations preincubated overnight in CA and SREG media were coincubated for 1 hour with previously salt-stored human zonae pellucidae also in the same media. Significantly more motile spermatozoa were bound to more of the zonae in CA medium (53.9% vs. 27.6% of zonae with 13.8 vs. 4.3 sperm/bound zona). In three hamster egg penetration test (HEPT) experiments, sperm populations preincubated overnight in either CA or SREG media were coincubated with hamster oocytes prepared in the same media. Only 2.1% of oocytes (1.0 polyspermy) were penetrated in SREG medium, cf., 30.9% of oocytes (1.3 polyspermy) in CA medium. These results demonstrate that while Sr2+ ions can substitute fully for Ca2+ in the capacitation and acrosome reaction of human spermatozoa, sperm-zona, and sperm-oolemma interaction seem to involve some more Ca2+-specific process(es). Furthermore, the increased HEPT fertilizing ability of human spermatozoa using overnight preincubation in SREG medium and CA medium for the test cannot be explained on the basis of differential kinetics of capacitation or the acrosome reaction.
D Mortimer; M J Chorney; E F Curtis; A O Trounson
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of experimental zoology     Volume:  246     ISSN:  0022-104X     ISO Abbreviation:  J. Exp. Zool.     Publication Date:  1988 May 
Date Detail:
Created Date:  1988-08-23     Completed Date:  1988-08-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0375365     Medline TA:  J Exp Zool     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  194-201     Citation Subset:  IM    
Department of Ob/Gyn, University of Calgary, Alberta, Canada.
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MeSH Terms
Acrosome / physiology
Calcium / pharmacology*
Culture Media
Fertilization / drug effects
Oocytes / physiology
Sperm Capacitation / drug effects
Sperm-Ovum Interactions / drug effects*
Spermatozoa / physiology*
Strontium / pharmacology*
Zona Pellucida / physiology
Reg. No./Substance:
0/Culture Media; 7440-24-6/Strontium; 7440-70-2/Calcium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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